不同吸附剂血液灌流对甲氨蝶呤清除的体外和离体评价。

In vitro and ex vivo evaluation of methotrexate removal by different sorbents haemoperfusion.

作者信息

Giardino R, Fini M, Giavaresi G, Spighi M, Faenza S, Orlandi M, Florio M L

机构信息

Anaesthesia Department, University of Bologna, Italy.

出版信息

Biomater Artif Cells Immobilization Biotechnol. 1993;21(4):447-54. doi: 10.3109/10731199309117650.

DOI:10.3109/10731199309117650

PMID:8260573

Abstract

The administration of high doses of Methotrexate (HDMTX) seems to be very effective in neoplastic treatment. Furthermore high doses of the drug could overcome the Methotrexate (MTX) resistance that it is possible to observe in some kind of cancers. To avoid several serious problems in the therapeutical application of HDMTX such as nephrotoxicity, hepatotoxicity and the difficulty to assess the right therapeutic range of the specific antagonist Leucovorin, a Haemoperfusion (HP) treatment could be very usefulL. In this study 3 anion exchange resins with different active group (trimethylammonium, dimethylethanolammonium and dimethylammina) and a spherical petroleum based charcoal were tested both in vitro and ex vivo to evaluate their capability to remove MTX. These results show a good strength and biocompatibility of the 4 sorbents and demonstrate the active group trimethylammonium resin as the most effective one.

摘要

大剂量甲氨蝶呤（HDMTX）给药在肿瘤治疗中似乎非常有效。此外，高剂量的该药物可以克服在某些癌症中可能观察到的甲氨蝶呤（MTX）耐药性。为避免HDMTX治疗应用中出现的一些严重问题，如肾毒性、肝毒性以及难以评估特定拮抗剂亚叶酸的正确治疗范围，血液灌流（HP）治疗可能非常有用。在本研究中，对3种具有不同活性基团（三甲基铵、二甲基乙醇铵和二甲基氨）的阴离子交换树脂以及一种球形石油基活性炭进行了体外和离体测试，以评估它们去除MTX的能力。这些结果显示了这4种吸附剂具有良好的强度和生物相容性，并证明三甲基铵树脂活性基团是最有效的一种。