Fini M, Spighi M, Piva D, Mongiorgi R, Capelli S, Nicoli Aldini N, Giardino R
Department of Experimental Surgery and Surgical Pathophysiology-IOR, University of Bologna.
Boll Soc Ital Biol Sper. 1993 Nov;69(11):683-9.
To evaluate the biocompatibility of an anionic exchange resin with active group trimethylammonium and charged with chloride ions an experimental study was performed with different tests to determine cytocompatibility, acute systemic toxicity, intracutaneous reactivity, allergic sensibilization, haemocompatibility and release of acetone, dichloromethane, quaternary amines and heavy metals (Pb, Cu, Cd) in eluate. Thus an anionic exchange resin can link all anionic substances; an ex vivo study on the participation of the dissociated sodic heparin in the anionic exchange was also conducted. In fact every time an extracorporeal circuit is made an anticoagulant must be administered to the patient and the removal of the drug could cause serious haemocoagulative problems. The results of the study showed a high biocompatibility of the resin that did not cause different significant reactions in comparison with negative control substances. The coating of the resin with poly-hydroxy-ethyl methacrilate with a separation phase technique avoided heparin removal from blood. Therefore adjustment of the dosage may not be necessary during further in vivo experiments. Future researches aiming to evaluate the the effectiveness and the biocompatibility of the sorbent in a living being will be performed in the next steps of the study.
为评估带有活性基团三甲基铵并负载氯离子的阴离子交换树脂的生物相容性，进行了一项实验研究，采用不同测试来确定细胞相容性、急性全身毒性、皮内反应性、过敏致敏性、血液相容性以及洗脱液中丙酮、二氯甲烷、季胺和重金属（铅、铜、镉）的释放情况。因此，一种阴离子交换树脂可以结合所有阴离子物质；还进行了一项关于解离的钠肝素参与阴离子交换的体外研究。事实上，每次建立体外循环时都必须给患者使用抗凝剂，而药物的清除可能会导致严重的血液凝固问题。研究结果表明该树脂具有高生物相容性，与阴性对照物质相比不会引起不同的显著反应。采用分离相技术用聚甲基丙烯酸羟乙酯对树脂进行包被可避免从血液中清除肝素。因此，在进一步的体内实验中可能无需调整剂量。旨在评估该吸附剂在生物体内有效性和生物相容性的未来研究将在该研究的后续步骤中进行。
