Yera Hélène, Andiva Shakir, Perret Catherine, Limonne Denis, Boireau Pascal, Dupouy-Camet Jean
Laboratoire de Parasitologie-Mycologie, Centre National de Référence des Trichinella Hôpital Cochin, Université R. Descartes, Assistance Publique-Hôpitaux de Paris, 75014 Paris, France.
Clin Diagn Lab Immunol. 2003 Sep;10(5):793-6. doi: 10.1128/cdli.10.5.793-796.2003.
We evaluated industrially prepared Western blot strips designed to avoid the cross-reactions observed with indirect immunofluorescence and enzyme-linked immunosorbent assays used for the serodiagnosis of trichinellosis. The antigen preparations were crude extracts of Trichinella spiralis. The Western blot profile characteristic of trichinellosis was characterized by comparing 60 sera from patients infected by Trichinella to 11 sera from healthy subjects, 51 sera from patients with other proven parasitic diseases (cysticercosis, schistosomiasis, strongyloidosis, fascioliasis, toxocariasis, liver amebiasis, anisakiasis, filariasis, toxoplasmosis, hydatidosis, or malaria), and 23 sera from patients with autoantibodies. Specific 43- to 44-kDa and 64-kDa bands were obtained with all of the sera from 51 patients with acute trichinellosis, in 4 out of 9 patients at the early stages of the disease, and in only 1 control patient, who had suspected anisakiasis and in whom trichinellosis could not be ruled out by muscle biopsy.
我们评估了工业制备的蛋白质印迹条带，这些条带旨在避免在旋毛虫病血清诊断中使用的间接免疫荧光法和酶联免疫吸附测定法所观察到的交叉反应。抗原制剂为旋毛虫的粗提物。通过比较60份旋毛虫感染患者的血清、11份健康受试者的血清、51份患有其他已确诊寄生虫病（囊尾蚴病、血吸虫病、类圆线虫病、肝片吸虫病、弓蛔虫病、肝阿米巴病、异尖线虫病、丝虫病、弓形虫病、包虫病或疟疾）患者的血清以及23份自身抗体患者的血清，对旋毛虫病特有的蛋白质印迹图谱特征进行了表征。在51例急性旋毛虫病患者的所有血清中、9例疾病早期患者中的4例以及仅1例疑似异尖线虫病且肌肉活检不能排除旋毛虫病的对照患者中，均获得了43至44 kDa和64 kDa的特异性条带。
