免疫复合物解离的p24抗原检测对婴儿早期检测人类免疫缺陷病毒的敏感性。
Sensitivity of immune complex-dissociated p24 antigen testing for early detection of human immunodeficiency virus in infants.
作者信息
Lewis D E, Adu-Oppong A, Hollinger F B, Rosenblatt H M, Hanson I C, Reuben J M, Kline M W, Kozinetz C A, Shearer W T
机构信息
Department of Microbiology, Baylor College of Medicine, Houston, Texas 77030.
出版信息
Clin Diagn Lab Immunol. 1995 Jan;2(1):87-90. doi: 10.1128/cdli.2.1.87-90.1995.
Abstract
Several investigators have suggested that early diagnosis of human immunodeficiency virus (HIV) infection in infants could be accomplished with a modified, more-sensitive, acid-dissociated p24 antigen enzyme-linked immunosorbent assay (ELISA) technique (p24 antigen immune complex dissociation [ICD]). We compared detection of HIV infection by HIV culture, PCR, and p24 antigen ICD assays in 46 infants by using samples collected independently. The detection sensitivity of the p24 antigen ICD assay was 0% with cord blood samples (2 HIV-positive infants), 38% with plasma samples from infants under 3 months of age (8 HIV-positive infants), and 58% overall (12 HIV-positive infants). By contrast, the sensitivities of HIV culture and PCR were 50% for cord blood samples, 75% for plasma samples from infants under 3 months of age, and 83% overall. These results indicate that the p24 antigen ICD does not offer the sensitivity necessary for this assay to be used as an indicator of HIV infection in infants.
摘要
几位研究人员提出，采用改良的、更灵敏的酸解离p24抗原酶联免疫吸附测定（ELISA）技术（p24抗原免疫复合物解离[ICD]）可实现对婴儿人类免疫缺陷病毒（HIV）感染的早期诊断。我们使用独立采集的样本，比较了46名婴儿中通过HIV培养、聚合酶链反应（PCR）和p24抗原ICD检测法对HIV感染的检测情况。p24抗原ICD检测法对脐带血样本（2名HIV阳性婴儿）的检测灵敏度为0%，对3个月龄以下婴儿的血浆样本（8名HIV阳性婴儿）的检测灵敏度为38%，总体检测灵敏度为58%（12名HIV阳性婴儿）。相比之下，HIV培养和PCR对脐带血样本的灵敏度为50%，对3个月龄以下婴儿的血浆样本的灵敏度为75%，总体灵敏度为83%。这些结果表明，p24抗原ICD不具备将该检测法用作婴儿HIV感染指标所必需的灵敏度。
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