Itoh K, Okubo K, Yosii J, Yokouchi H, Matsubara K
Institute for Molecular and Cellular Biology, Osaka University, Japan.
DNA Res. 1994;1(6):279-87. doi: 10.1093/dnares/1.6.279.
An expression profile of genes active in the human lung was obtained by collecting 797 partial sequences from a 3'-directed cDNA library. Three genes were found to produce mRNA each of which comprised more than 1% of total mRNA. These three have been identified as genes for pulmonary surfactant apoprotein (PSP-A), Clara cells 10-kDa secretory protein, and HLA-E heavy chain. In the remaining 745 clones, 221 were composed of 89 species that occurred recurrently, and 524 clones appeared only once. Because the 3'-directed cDNA library faithfully represents the mRNA population in the source tissue, these numbers represent the relative activities of the gene expression. Altogether 437 gene species were novel, and 179 gene species were identified in GenBank. A significant portion of these genes encode proteins found in secretory proteins, cell surface proteins, and components in the protein synthesis machinery, representing the function of the lung.
通过从一个3'端定向的cDNA文库中收集797个部分序列，获得了在人肺中活跃的基因表达谱。发现有三个基因产生的mRNA各自占总mRNA的1%以上。这三个基因已被鉴定为肺表面活性物质载脂蛋白（PSP-A）、克拉拉细胞10 kDa分泌蛋白和HLA-E重链的基因。在其余745个克隆中，221个由89种反复出现的物种组成，524个克隆只出现过一次。由于3'端定向的cDNA文库忠实地代表了源组织中的mRNA群体，这些数字代表了基因表达的相对活性。总共437个基因种类是新的，179个基因种类在GenBank中被鉴定出来。这些基因中有很大一部分编码在分泌蛋白、细胞表面蛋白和蛋白质合成机制中的成分中发现的蛋白质，代表了肺的功能。
