Okubo K, Yoshii J, Yokouchi H, Kameyama M, Matsubara K
Institute for Molecular and Cellular Biology, Osaka University, Japan.
DNA Res. 1994;1(1):37-45. doi: 10.1093/dnares/1.1.37.
An expression profile of genes active in the human colonic mucosa was obtained by collecting 959 partial sequences from a 3'-directed cDNA library. Seven genes were found to produce mRNA each of which comprised more than 1% of total mRNA. Four of these genes are novel, and are likely to be uniquely expressed in the colonic mucosa, and the other three have been identified as genes for fatty acid binding protein, immunoglobulin lambda chain, and carcinoma-associated antigen GA733-2. In the remaining 952 clones, 310 were composed of 118 species occurred recurrently but less than 1%, and 533 clones appeared only once. Because the 3'-directed cDNA library faithfully represents the mRNA population in the source tissue, these numbers represent the relative activities of the gene expression. Altogether 156 gene species were identified in GenBank, and a significant portion of these genes encode proteins found in Golgi apparatus and lysosomes, chromosome-encoded mitochondrial proteins, cell surface proteins, and components in the protein synthesis machinery. The types and proportions of genes identified is consistent with the known major activities of the colonic mucosa such as mucous protein production, energy-dependent water absorption, and rapid cell proliferation and turnover.
通过从一个3'端定向的cDNA文库中收集959个部分序列，获得了在人类结肠黏膜中活跃的基因表达谱。发现有7个基因产生的mRNA各自占总mRNA的1%以上。其中4个基因为新基因，可能在结肠黏膜中独特表达，另外3个已被鉴定为脂肪酸结合蛋白、免疫球蛋白λ链和癌相关抗原GA733 - 2的基因。在其余952个克隆中，310个由118种反复出现但低于1%的序列组成，533个克隆只出现过一次。由于3'端定向的cDNA文库忠实地反映了来源组织中的mRNA群体，这些数字代表了基因表达的相对活性。在GenBank中总共鉴定出156种基因，这些基因中有很大一部分编码在高尔基体和溶酶体中发现的蛋白质、染色体编码的线粒体蛋白质、细胞表面蛋白质以及蛋白质合成机制中的成分。所鉴定基因的类型和比例与结肠黏膜已知的主要活动一致，如黏液蛋白产生、能量依赖的水吸收以及快速的细胞增殖和更新。
