Meng F, Chen Y, Lu C
Burn Institute, Shanghai Hospital, Second Military Medical University.
Zhonghua Zheng Xing Shao Shang Wai Ke Za Zhi. 1994 Nov;10(6):447-51.
The dynamic properties of membrane protein are closely correlated to the membrane functions in erythrocytes. The anion transport function of band 3 protein, the constituents and the translational diffusion of membrane protein was detected in 25% TBSA burn rats. The changes in membrane protein SH were investigated by using the spin label of 3-maleimide-proxyl. The results showed that, in delayed fluid resuscitation group after burn shock, membrane crosslinking protein appeared in erythrocytes, the function of band 3 protein was damaged, the recovery time after fluorescence photobleaching of membrane protein was obviously prolonged, and the ratio of peak heights of strong to weak immobilization was increased, especially in the six-hour delayed resuscitation group, suggesting that the ischemia-reperfusion injury was more serious during the early shock stage. The authors believe that the results may represent the mechanism of damage biological membrane after burns. The alterations of membrane properties caused by free radicals play an important role in erythrocyte injury.
膜蛋白的动态特性与红细胞的膜功能密切相关。在25%体表面积烧伤大鼠中检测了带3蛋白的阴离子转运功能、膜蛋白的组成成分及平移扩散。采用3-马来酰亚胺-脯氨酰自旋标记法研究膜蛋白SH的变化。结果显示，烧伤休克延迟液体复苏组红细胞出现膜交联蛋白，带3蛋白功能受损，膜蛋白荧光漂白后的恢复时间明显延长，强固定与弱固定峰高比值增加，尤其是在延迟复苏6小时组，提示休克早期缺血再灌注损伤更严重。作者认为该结果可能代表烧伤后生物膜损伤的机制。自由基引起的膜特性改变在红细胞损伤中起重要作用。
