Niwamoto H, Okamoto E, Fujimoto J, Takeuchi M, Furuyama J, Yamamoto Y
First Department of Surgery, Hyogo College of Medicine, Japan.
Dig Dis Sci. 1995 Apr;40(4):859-64. doi: 10.1007/BF02064992.
In order to test the hypothesis that esophageal achalasia may be due to neurotropic viral damage to the esophageal myenteric plexus, esophageal tissue with or without achalasia was analyzed by polymerase chain reaction for the presence of human herpes virus DNA or measles virus RNA. The DNA and RNA were extracted from the esophageal muscle of 12 patients with achalasia and six patients with upper esophageal carcinoma. Peripheral blood mononuclear cells from eight adult volunteers and two samples of umbilical blood mononuclear cells were also used as controls. PCR amplification with a pair of primers specific for herpes simplex type 1 and 2 viruses identified 92-bp fragments in nearly all specimens, including those without achalasia. Each 92-bp fragment was confirmed to be identical to a single herpes simplex virus sequence by automated DNA sequence analysis. No amplification for five other herpes viruses or measles virus was detected. Therefore, a specific viral etiology for achalasia was not identified in this study.
为了检验食管失弛缓症可能是由于嗜神经病毒对食管肌间神经丛造成损害这一假说，运用聚合酶链反应分析了有或没有失弛缓症的食管组织，以检测人疱疹病毒DNA或麻疹病毒RNA的存在情况。从12例失弛缓症患者和6例食管上段癌患者的食管肌肉中提取DNA和RNA。来自8名成年志愿者的外周血单个核细胞以及两份脐血单个核细胞样本也用作对照。用一对针对1型和2型单纯疱疹病毒的特异性引物进行PCR扩增，在几乎所有标本中都鉴定出了92bp的片段，包括那些没有失弛缓症的标本。通过自动DNA序列分析证实，每个92bp片段都与单一的单纯疱疹病毒序列相同。未检测到其他5种疱疹病毒或麻疹病毒的扩增。因此，本研究未确定失弛缓症的特定病毒病因。
