Sanders V J, Felisan S, Waddell A, Tourtellotte W W
Department of Neurology, UCLA School of Medicine 90095, USA.
J Neurovirol. 1996 Aug;2(4):249-58. doi: 10.3109/13550289609146888.
To test for the presence of herpesviruses in postmortem brain samples from multiple sclerosis patients and controls using polymerase chain reaction.
Herpes simplex virus, varicella-zoster virus, Epstein-Barr virus, cytomegalovirus, and human herpesvirus-6 are common viruses capable of persistence and latency. All have been detected in the CNS.
Active and inactive plaque tissue, unaffected white matter (WM) and gray matter (GM) from MS cases, and WM and GM controls (Alzheimer's disease, Parkinson's disease and non-neurological disease) were screened for the herpesvirus by PCR.
(1) 37% of the MS cases were positive for herpes simplex virus (HSV). Twenty-eight percent of controls cases were positive for HSV. Forty-one percent of active plaques were positive for HSV in contrast to only 20% of inactive plaques (Sanders et al, 1996). (2) 57% of the MS cases and 43% of the control cases were positive for HHV-6. Thirty-two percent of the active plaques contained HHV-6 compared to 17% of inactive plaques. (3) 43% of the MS cases and 32% of the control cases were positive for VZV. Fourteen percent of the active plaques and 10% of the inactive plaques were positive for VZV. (4) 27% of MS cases and 38% of control cases were positive for EBV. Five percent of the active plaques were positive for EBV and 10% of the inactive plaques were positive. (5) 16% of the MS cases and 22% of the controls were positive for CMV. Nine percent of the active plaques and 10% of the inactive plaques were positive. We also compared MS WM and GM with controls and found no significant difference.
HSV, HHV-6, and VZV were present in a greater frequency of MS cases compared to controls; however, no statistical differences were noted. The presence of herpesvirus in all tissue makes an etiologic association to MS uncertain. Cellular localization of virus and its relationship to pathology and latency may reveal an association.
使用聚合酶链反应检测多发性硬化症患者和对照者的尸检脑样本中是否存在疱疹病毒。
单纯疱疹病毒、水痘-带状疱疹病毒、爱泼斯坦-巴尔病毒、巨细胞病毒和人类疱疹病毒6型是能够持续存在和潜伏的常见病毒。所有这些病毒都已在中枢神经系统中被检测到。
通过聚合酶链反应对多发性硬化症病例的活动期和静止期斑块组织、未受影响的白质和灰质，以及白质和灰质对照（阿尔茨海默病、帕金森病和非神经疾病）进行疱疹病毒筛查。
（1）37%的多发性硬化症病例单纯疱疹病毒（HSV）呈阳性。28%的对照病例HSV呈阳性。41%的活动期斑块HSV呈阳性，而静止期斑块仅20%呈阳性（桑德斯等人，1996年）。（2）57%的多发性硬化症病例和43%的对照病例人类疱疹病毒6型（HHV-6）呈阳性。32%的活动期斑块含有HHV-6，而静止期斑块为17%。（3）43%的多发性硬化症病例和32%的对照病例水痘-带状疱疹病毒（VZV）呈阳性。14%的活动期斑块和10%的静止期斑块VZV呈阳性。（4）27%的多发性硬化症病例和38%的对照病例爱泼斯坦-巴尔病毒（EBV）呈阳性。5%的活动期斑块EBV呈阳性，10%的静止期斑块呈阳性。（5）16%的多发性硬化症病例和22%的对照病例巨细胞病毒（CMV）呈阳性。9%的活动期斑块和10%的静止期斑块呈阳性。我们还将多发性硬化症的白质和灰质与对照进行了比较，未发现显著差异。
与对照相比，多发性硬化症病例中HSV、HHV-6和VZV的出现频率更高；然而，未观察到统计学差异。所有组织中疱疹病毒的存在使得其与多发性硬化症的病因学关联不确定。病毒的细胞定位及其与病理学和潜伏期的关系可能揭示一种关联。
