Käsbohrer A, Liebisch G, Schönberg A, Liebisch A
WHO Collaborating Centre for Research and Training in Veterinary Public Health, Institut für Biometrie u. Epidemiologie, Tierärztliche Hochschule Hannover.
Dtsch Tierarztl Wochenschr. 1994 Dec;101(12):476-81.
The aim of the study was to develop and validate a serological test system for extended epidemiological investigations to which extend dogs were exposed to Borrelia burgdorferi. For this purpose, 121 samples of dogs which were suspect of an infection and submitted to the laboratory for serological testing, were investigated in an immunofluorescence test (IFT) and to different enzyme-linked immunosorbent assays (ELISA). Valuation of the ELISA systems was assessed in relation to the IFT. Sensitivity, specificity and predictive values were calculated for negative, positive and also borderline values. In the screening test all samples with a positive titre in IFT were judged positive. Samples negative in IFT showed negative results in the screening in only 86%. All samples positive in screening or of borderline value in IFT were again tested using a confirmatory ELISA. By this procedure a specificity of 100% and a sensitivity of 85% was calculated for samples with positive or negative IFT titres, respectively. Samples with IFT borderline titres (1:64 or 1:128) were judged negative in this ELISA in 80% and borderline in 16%. Checking of selected samples in an immunodot test confirmed the ELISA results. Sera being negative in ELISA showed also no specific reaction in this test. When considering the possibility of false positive reactions in IFT, rather high percentages of sensitivity and specificity could be found. Because all real positive samples could be detected using the confirmatory ELISA as a single test, there is no further need in epidemiological investigations to use the screening test. For specific problems the Western blot can be used.(ABSTRACT TRUNCATED AT 250 WORDS)
该研究的目的是开发并验证一种血清学检测系统，用于对接触伯氏疏螺旋体的犬类进行扩大的流行病学调查。为此，对121份疑似感染并提交至实验室进行血清学检测的犬类样本，采用免疫荧光试验（IFT）和不同的酶联免疫吸附测定（ELISA）进行了研究。ELISA系统的评估是相对于IFT进行的。计算了阴性、阳性以及临界值的敏感性、特异性和预测值。在筛查试验中，IFT中滴度为阳性的所有样本均判定为阳性。IFT中呈阴性的样本在筛查中仅86%显示阴性结果。所有筛查呈阳性或IFT临界值的样本再次使用确证ELISA进行检测。通过该程序，对于IFT滴度为阳性或阴性的样本，分别计算出特异性为100%，敏感性为85%。IFT临界滴度（1:64或1:128）的样本在该ELISA中80%判定为阴性，16%判定为临界值。在免疫斑点试验中对选定样本进行检查，证实了ELISA结果。ELISA中呈阴性的血清在该试验中也未显示特异性反应。考虑到IFT中假阳性反应的可能性，可以发现相当高的敏感性和特异性百分比。由于使用确证ELISA作为单一检测可以检测到所有真正的阳性样本，因此在流行病学调查中无需再使用筛查试验。对于特定问题，可以使用蛋白质印迹法。（摘要截短于250字）
