Nilsen H, Yazdankhah S P, Eftedal I, Krokan H E
UNIGEN Center for Molecular Biology, University of Trondheim, Norway.
FEBS Lett. 1995 Apr 3;362(2):205-9. doi: 10.1016/0014-5793(95)00244-4.
The rate of removal of uracil from different positions in double-stranded DNA by uracil-DNA glycosylase from Escherichia coli varied more than 15-fold. Consensus sequences for good and poor removal were 5'-(A/T)UA(A/T)-3' and 5'-(G/C)U(T/G/C)-3', respectively. In general, the sequence context surrounding U was more important for the rate of removal than whether U was present in U.A pairs or U.G mispairs. Rates of removal of U from sites of amber mutations in the lacI gene, where mutation frequencies and deamination rates were known, indicated that the observed variation in removal is biologically significant.
来自大肠杆菌的尿嘧啶-DNA糖基化酶从双链DNA不同位置去除尿嘧啶的速率变化超过15倍。去除效果好和差的共有序列分别为5'-(A/T)UA(A/T)-3'和5'-(G/C)U(T/G/C)-3'。一般来说，尿嘧啶周围的序列环境对去除速率比尿嘧啶是存在于U·A碱基对还是U·G错配中更为重要。从已知突变频率和脱氨基速率的lacI基因琥珀突变位点去除尿嘧啶的速率表明，观察到的去除差异具有生物学意义。
