Douglas Katherine, Sorensen Dana, Saud Arnav, Sridharan Ananya, Mathew Mallika, Hiranandani Jyotsna, Gonick Ava, Young Courtney, Nguyen Kelly, Phi Nhu, Miller Jeffrey H
Department of Microbiology, Immunology, and Molecular Genetics, and the Molecular Biology Institute, University of California, and the David Geffen School of Medicine, Los Angeles, California, USA.
Environ Mol Mutagen. 2025 Aug;66(6-7):327-340. doi: 10.1002/em.70021. Epub 2025 Jun 22.
We constructed a catalog of mutational sites in the tdk gene of Escherichia coli that consists of 378 different base pair substitutions at 245 different sites (base pairs). This allows us to examine the tendency of different sub-regions of the gene to be more or less prone to mutations when compared with other sub-regions. We do this by recording the number of occurrences of close to 1100 mutations resulting from 9 different mutagens or mutators at each site within a rolling 20-site interval. We can define 5 mutationally prone regions (MPRs). Aligning the profile of MPRs in tdk next to those we previously described in the thyA gene results in a linear map with 8 MPR regions, reinforcing the concept that certain sub-regions of genes are more mutable than others, even when other variables are accounted for. The catalog should prove useful for future studies.
我们构建了大肠杆菌tdk基因的突变位点目录，该目录包含245个不同位点（碱基对）的378种不同碱基对替换。这使我们能够研究该基因不同子区域与其他子区域相比发生突变的倾向是更高还是更低。我们通过记录在滚动的20个位点区间内，每个位点由9种不同诱变剂或诱变因素导致的近1100个突变的出现次数来实现这一点。我们可以定义5个突变倾向区域（MPR）。将tdk基因中的MPR图谱与我们之前在thyA基因中描述的图谱对齐，会得到一个包含8个MPR区域的线性图谱，这强化了一个概念，即基因的某些子区域比其他子区域更易发生突变，即使考虑了其他变量。该目录应会对未来的研究有用。
