Anthony B F
J Exp Med. 1976 May 1;143(5):1186-98. doi: 10.1084/jem.143.5.1186.
The opsonization and phagocytosis of group B streptococci of types Ia, Ib, and Ic were studied in vitro by measuring the uptake of radioactivity by coverslip cultures of rabbit alevolar macrophages during incubation with radiolabeled, nonviable bacteria which had been exposed to rabbit serum. The uptake of counts per minute was quantitative, reproducible, and reversibly inhibited by cold, indicating that it was largely a measurement of phagocytic ingestion rather than of attachment of bacteria-immunoglobulin complexes to macrophage membranes. Moreover, suspended macrophages killed approximately 90% of viable streptococci in the presence of specific antiserum. The opsonic activity of immune serum was heat stable, and phagocytosis of streptococci was insignificant after incubation with normal serum and antiserum to some heterologous group B streptococci. By absorption studies, it was possible to identify the effect of antibodies to specific bacterial antigens. Phagocytosis of streptococci containing the corresponding antigens was maximal after opsonization with homologous or heterologous sera containing antibody to IaCHO, IbCHO, or Ibc protein. Phagocytosis of all three serotypes was intermediate when opsonization could be attributed to anti-IabcCHO. The opsonization of a specific group B streptococcus is complex and may involve two or more antigen-antibody systems.
通过在与经兔血清处理的放射性标记的非存活细菌孵育期间，测量兔肺泡巨噬细胞盖玻片培养物的放射性摄取，对Ia、Ib和Ic型B族链球菌的调理作用和吞噬作用进行了体外研究。每分钟计数的摄取是定量的、可重复的，并且可被冷可逆抑制，这表明它主要是吞噬摄取的测量，而不是细菌 - 免疫球蛋白复合物与巨噬细胞膜的附着的测量。此外，在存在特异性抗血清的情况下，悬浮的巨噬细胞杀死了大约90%的存活链球菌。免疫血清的调理活性是热稳定的，并且在用正常血清和针对某些异源B族链球菌的抗血清孵育后，链球菌的吞噬作用不明显。通过吸收研究，可以确定针对特定细菌抗原的抗体的作用。在用含有针对IaCHO、IbCHO或Ibc蛋白的抗体的同源或异源血清调理后，含有相应抗原的链球菌的吞噬作用最大。当调理作用可归因于抗IabcCHO时，所有三种血清型的吞噬作用处于中等水平。特定B族链球菌的调理作用是复杂的，可能涉及两个或更多个抗原 - 抗体系统。
