Horne D, Tomasz A
Antimicrob Agents Chemother. 1981 May;19(5):745-53. doi: 10.1128/AAC.19.5.745.
Pretreatment of serotype Ib group B streptococci with benzylpenicillin, other beta-lactam antibiotics, or vancomycin increased the susceptibility of these bacteria to the bactericidal activity of a mixture of human polymorphonuclear leukocytes and normal human serum. Increased susceptibility of the bacteria to killing by phagocytes was elicited even by exposure to subinhibitory levels of the beta-lactam antibiotics. Inhibitors of protein synthesis did not induce such susceptibility. We investigated the possible biochemical basis of penicillin-induced susceptibility to phagocytosis. Penicillin treatment induced the release of substantial quantities of group B streptococcal surface components into the growth medium (lipoteichoic acid, lipid, and capsular polysaccharide). Labeling of the live streptococci with 3H-labeled penicillin was used to evaluate the effect of exposure to subinhibitory concentrations of this antibiotic on the penicillin-binding proteins. Our results suggested that beta-lactam antibiotics and components of the immune system may act in concert to eliminate invading bacteria.
用苄青霉素、其他β-内酰胺类抗生素或万古霉素对B族链球菌血清型Ib进行预处理，可增加这些细菌对人多形核白细胞与正常人血清混合物杀菌活性的敏感性。即使暴露于亚抑制水平的β-内酰胺类抗生素，也会引发细菌对吞噬细胞杀伤的敏感性增加。蛋白质合成抑制剂不会诱导这种敏感性。我们研究了青霉素诱导的吞噬易感性的可能生化基础。青霉素处理导致大量B族链球菌表面成分释放到生长培养基中（脂磷壁酸、脂质和荚膜多糖）。用3H标记的青霉素标记活链球菌，以评估暴露于亚抑制浓度的这种抗生素对青霉素结合蛋白的影响。我们的结果表明，β-内酰胺类抗生素与免疫系统成分可能协同作用以清除入侵细菌。
