淋病奈瑟菌主要铁调节外膜蛋白FrpB编码基因的克隆、测序及特性分析
Cloning, sequencing, and characterization of the gene encoding FrpB, a major iron-regulated, outer membrane protein of Neisseria gonorrhoeae.
作者信息
Beucher M, Sparling P F
机构信息
Department of Medicine, School of Medicine, University of North Carolina at Chapel Hill 27599, USA.
出版信息
J Bacteriol. 1995 Apr;177(8):2041-9. doi: 10.1128/jb.177.8.2041-2049.1995.
Abstract
FrpB (for Fe-regulated protein B) is a 76-kDa outer membrane protein that is part of the iron regulon of Neisseria gonorrhoeae and Neisseria meningitidis. The frpB gene from gonococcal strain FA19 was cloned and sequenced. FrpB was homologous to several TonB-dependent outer membrane receptors of Escherichia coli as well as HemR of Yersinia enterocolitica and CopB of Moraxella catarrhalis. An omga insertion into the frpB coding sequence caused a 60% reduction in 55Fe uptake from heme, but careful analysis suggested that this effect was nonspecific. While FrpB was related to the family of TonB-dependent proteins, a function in iron uptake could not be documented.
摘要
FrpB（铁调节蛋白B）是一种76千道尔顿的外膜蛋白，是淋病奈瑟菌和脑膜炎奈瑟菌铁调节子的一部分。对淋病奈瑟菌菌株FA19的frpB基因进行了克隆和测序。FrpB与大肠杆菌的几种TonB依赖性外膜受体、小肠结肠炎耶尔森菌的HemR以及卡他莫拉菌的CopB具有同源性。在frpB编码序列中插入一个ω元件导致从血红素摄取55Fe的量减少了60%，但仔细分析表明这种效应是非特异性的。虽然FrpB与TonB依赖性蛋白家族有关，但在铁摄取中的功能尚无记录。
相似文献
1
Cloning, sequencing, and characterization of the gene encoding FrpB, a major iron-regulated, outer membrane protein of Neisseria gonorrhoeae.淋病奈瑟菌主要铁调节外膜蛋白FrpB编码基因的克隆、测序及特性分析
J Bacteriol. 1995 Apr;177(8):2041-9. doi: 10.1128/jb.177.8.2041-2049.1995.
2
Gonococcal transferrin-binding protein 1 is required for transferrin utilization and is homologous to TonB-dependent outer membrane receptors.淋球菌转铁蛋白结合蛋白1是利用转铁蛋白所必需的，并且与TonB依赖性外膜受体同源。
J Bacteriol. 1992 Sep;174(18):5788-97. doi: 10.1128/jb.174.18.5788-5797.1992.
3
Sequence variability of FrpB, a major iron-regulated outer-membrane protein in the pathogenic neisseriae.致病奈瑟菌中主要的铁调节外膜蛋白FrpB的序列变异性。
Microbiology (Reading). 1996 Nov;142 ( Pt 11):3269-74. doi: 10.1099/13500872-142-11-3269.
4
Hemin uptake system of Yersinia enterocolitica: similarities with other TonB-dependent systems in gram-negative bacteria.小肠结肠炎耶尔森菌的血红素摄取系统：与革兰氏阴性菌中其他依赖TonB的系统的相似性
EMBO J. 1992 Dec;11(12):4359-67. doi: 10.1002/j.1460-2075.1992.tb05535.x.
5
Molecular cloning and characterization of a 79-kDa iron-repressible outer-membrane protein of Moraxella bovis.牛莫拉菌79-kDa铁抑制性外膜蛋白的分子克隆与特性分析
FEMS Microbiol Lett. 2003 Aug 29;225(2):279-84. doi: 10.1016/S0378-1097(03)00554-8.
6
Molecular characterization of FrpB, the 70-kilodalton iron-regulated outer membrane protein of Neisseria meningitidis.脑膜炎奈瑟菌70千道尔顿铁调节外膜蛋白FrpB的分子特征
Infect Immun. 1995 Oct;63(10):4181-4. doi: 10.1128/iai.63.10.4181-4184.1995.
7
Cloning and functional characterization of Neisseria gonorrhoeae tonB, exbB and exbD genes.淋病奈瑟菌tonB、exbB和exbD基因的克隆及功能特性分析
Mol Microbiol. 1997 Apr;24(1):169-79. doi: 10.1046/j.1365-2958.1997.3421692.x.
8
A putative iron-regulated TonB-dependent receptor of Mannheimia (Pasteurella) haemolytica A1: possible mechanism for phase variation.溶血曼氏杆菌（巴斯德氏菌）A1中一种假定的铁调节TonB依赖性受体：相变的可能机制。
Vet Microbiol. 2002 Jan 3;84(1-2):53-67. doi: 10.1016/s0378-1135(01)00415-1.
9
Molecular cloning and characterization of the structural gene for the major iron-regulated protein expressed by Neisseria gonorrhoeae.淋病奈瑟菌表达的主要铁调节蛋白结构基因的分子克隆与特性分析
J Exp Med. 1990 May 1;171(5):1535-46. doi: 10.1084/jem.171.5.1535.
10
The Neisseria meningitidis haemoglobin receptor: its role in iron utilization and virulence.脑膜炎奈瑟菌血红蛋白受体：其在铁利用和毒力中的作用。
Mol Microbiol. 1995 Feb;15(3):531-41. doi: 10.1111/j.1365-2958.1995.tb02266.x.
引用本文的文献
1
Proteomics-driven Antigen Discovery for Development of Vaccines Against Gonorrhea.蛋白质组学驱动的抗原发现用于开发抗淋病疫苗
Mol Cell Proteomics. 2016 Jul;15(7):2338-55. doi: 10.1074/mcp.M116.058800. Epub 2016 May 2.
2
Characterization of vaccine antigens of meningococcal serogroup W isolates from Ghana and Burkina Faso from 2003 to 2009.2003年至2009年来自加纳和布基纳法索的W群脑膜炎球菌分离株疫苗抗原的特性分析
F1000Res. 2014 Nov 3;3:264. doi: 10.12688/f1000research.3881.1. eCollection 2014.
3
Antigen sequence typing of outer membrane protein (fetA) gene of Neisseria meningitidis serogroup A from Delhi & adjoining areas.德里及周边地区A群脑膜炎奈瑟菌外膜蛋白（fetA）基因的抗原序列分型
Indian J Med Res. 2014 Dec;140(6):766-9.
4
A genome-wide identification of genes undergoing recombination and positive selection in Neisseria.淋病奈瑟菌中经历重组和正选择的基因的全基因组鉴定。
Biomed Res Int. 2014;2014:815672. doi: 10.1155/2014/815672. Epub 2014 Aug 10.
5
Use of a molecular decoy to segregate transport from antigenicity in the FrpB iron transporter from Neisseria meningitidis.利用分子诱饵将脑膜炎奈瑟菌 FrpB 铁转运蛋白的运输功能与其抗原性分离。
PLoS One. 2013;8(2):e56746. doi: 10.1371/journal.pone.0056746. Epub 2013 Feb 15.
6
Refolding, purification and crystallization of the FrpB outer membrane iron transporter from Neisseria meningitidis.脑膜炎奈瑟菌FrpB外膜铁转运蛋白的重折叠、纯化及结晶
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Feb 1;68(Pt 2):231-5. doi: 10.1107/S1744309111056028. Epub 2012 Jan 27.
7
The iron-repressed, AraC-like regulator MpeR activates expression of fetA in Neisseria gonorrhoeae.铁抑制型 AraC 样调控因子 MpeR 激活淋病奈瑟菌 fetA 的表达。
Infect Immun. 2011 Dec;79(12):4764-76. doi: 10.1128/IAI.05806-11. Epub 2011 Sep 26.
8
TonB-Dependent Transporters Expressed by Neisseria gonorrhoeae.淋病奈瑟菌表达的TonB依赖性转运蛋白
Front Microbiol. 2011 May 27;2:117. doi: 10.3389/fmicb.2011.00117. eCollection 2011.
9
The fbpABC operon is required for Ton-independent utilization of xenosiderophores by Neisseria gonorrhoeae strain FA19.该 fbpABC 操纵子是淋病奈瑟菌 FA19 株利用异种铁载体所必需的，与 Ton 系统无关。
Infect Immun. 2011 Jan;79(1):267-78. doi: 10.1128/IAI.00807-10. Epub 2010 Nov 1.
10
TonB-dependent transporters and their occurrence in cyanobacteria.依赖TonB的转运蛋白及其在蓝细菌中的存在情况。
BMC Biol. 2009 Oct 12;7:68. doi: 10.1186/1741-7007-7-68.
本文引用的文献
1
NEISSERIA GONORRHOEAE. I. VIRULENCE GENETICALLY LINKED TO CLONAL VARIATION.淋病奈瑟菌。一、与克隆变异基因连锁的毒力。
J Bacteriol. 1963 Jun;85(6):1274-9. doi: 10.1128/jb.85.6.1274-1279.1963.
2
Phase variation of H. influenzae fimbriae: transcriptional control of two divergent genes through a variable combined promoter region.流感嗜血杆菌菌毛的相变：通过可变组合启动子区域对两个反向基因进行转录调控。
Cell. 1993 Jun 18;73(6):1187-96. doi: 10.1016/0092-8674(93)90647-9.
3
Neisseria meningitidis produces iron-regulated proteins related to the RTX family of exoproteins.脑膜炎奈瑟菌产生与外毒素RTX家族相关的铁调节蛋白。
J Bacteriol. 1993 Feb;175(3):811-8. doi: 10.1128/jb.175.3.811-818.1993.
4
Molecular characterization of the 98-kilodalton iron-regulated outer membrane protein of Neisseria meningitidis.脑膜炎奈瑟菌98千道尔顿铁调节外膜蛋白的分子特征
Infect Immun. 1993 Nov;61(11):4724-33. doi: 10.1128/iai.61.11.4724-4733.1993.
5
Identification and cloning of a fur homolog from Neisseria gonorrhoeae.淋病奈瑟菌中一个铁摄取调节蛋白同源物的鉴定与克隆。
Infect Immun. 1993 Nov;61(11):4599-606. doi: 10.1128/iai.61.11.4599-4606.1993.
6
Cloning and characterization of the Vibrio cholerae genes encoding the utilization of iron from haemin and haemoglobin.霍乱弧菌编码利用血红素和血红蛋白中铁元素的基因的克隆与特性分析。
Mol Microbiol. 1993 Feb;7(3):461-9. doi: 10.1111/j.1365-2958.1993.tb01137.x.
7
A mutation affecting expression of a major outer membrane protein of Moraxella catarrhalis alters serum resistance and survival in vivo.一种影响卡他莫拉菌主要外膜蛋白表达的突变会改变血清抗性及体内存活率。
J Infect Dis. 1993 Nov;168(5):1194-201. doi: 10.1093/infdis/168.5.1194.
8
Identification and cloning of a fur homologue from Neisseria meningitidis.从脑膜炎奈瑟菌中鉴定并克隆铁摄取调节蛋白（Fur）同源物
Mol Microbiol. 1994 Feb;11(4):725-37. doi: 10.1111/j.1365-2958.1994.tb00350.x.
9
Gonococcal transferrin-binding protein 2 facilitates but is not essential for transferrin utilization.淋球菌转铁蛋白结合蛋白2有助于转铁蛋白的利用，但并非必不可少。
J Bacteriol. 1994 Jun;176(11):3162-70. doi: 10.1128/jb.176.11.3162-3170.1994.
10
The role of encapsulated anaerobic bacteria in synergistic infections.被膜厌氧细菌在协同感染中的作用。
FEMS Microbiol Rev. 1994 Jan;13(1):65-74. doi: 10.1111/j.1574-6976.1994.tb00035.x.
Zotero 插件