不可或缺的大肠杆菌era基因的跨物种互补突显了活性所必需的氨基酸区域。
Cross-species complementation of the indispensable Escherichia coli era gene highlights amino acid regions essential for activity.
作者信息
Pillutla R C, Sharer J D, Gulati P S, Wu E, Yamashita Y, Lerner C G, Inouye M, March P E
机构信息
University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway 08854, USA.
出版信息
J Bacteriol. 1995 Apr;177(8):2194-6. doi: 10.1128/jb.177.8.2194-2196.1995.
Abstract
Era is an essential GTP binding protein in Escherichia coli. Two homologs of this protein, Sgp from Streptococcus mutans and Era from Coxiella burnetii, can substitute for the essential function of Era in E. coli. Site-specific and randomly generated Era mutants which may indicate regions of the protein that are of functional importance are described.
摘要
Era是大肠杆菌中一种重要的GTP结合蛋白。该蛋白的两个同源物，变形链球菌的Sgp和伯氏考克斯氏体的Era，可以替代大肠杆菌中Era的基本功能。本文描述了可能指示该蛋白功能重要区域的位点特异性和随机产生的Era突变体。
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本文引用的文献
1
Molecular characterization of a STreptococcus mutans mutant altered in environmental stress responses.变形链球菌环境应激反应改变突变株的分子特征分析
J Bacteriol. 1993 Oct;175(19):6220-8. doi: 10.1128/jb.175.19.6220-6228.1993.
2
Characterization of the autophosphorylation of Era, an essential Escherichia coli GTPase.Era（一种必需的大肠杆菌GTP酶）自身磷酸化的特性研究
Mol Microbiol. 1994 Apr;12(2):201-8. doi: 10.1111/j.1365-2958.1994.tb01009.x.
3
Genes in S. cerevisiae encoding proteins with domains homologous to the mammalian ras proteins.酿酒酵母中编码与哺乳动物ras蛋白具有同源结构域的蛋白质的基因。
Cell. 1984 Mar;36(3):607-12. doi: 10.1016/0092-8674(84)90340-4.
4
5
A GTP-binding protein of Escherichia coli has homology to yeast RAS proteins.
Proc Natl Acad Sci U S A. 1986 Dec;83(23):8849-53. doi: 10.1073/pnas.83.23.8849.
6
The Escherichia coli Ras-like protein (Era) has GTPase activity and is essential for cell growth.
Oncogene. 1988 Jun;2(6):539-44.
7
Molecular analysis of the Escherichia coli recO gene.
J Bacteriol. 1989 Jul;171(7):3641-9. doi: 10.1128/jb.171.7.3641-3649.1989.
8
Genetic analysis of the rnc operon of Escherichia coli.大肠杆菌rnc操纵子的遗传分析。
J Bacteriol. 1989 May;171(5):2581-90. doi: 10.1128/jb.171.5.2581-2590.1989.
9
Expression and characterization of RNase III and Era proteins. Products of the rnc operon of Escherichia coli.核糖核酸酶III和Era蛋白的表达与特性：大肠杆菌rnc操纵子的产物
J Biol Chem. 1990 Feb 15;265(5):2888-95.
10
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J Bacteriol. 1991 Apr;173(7):2265-70. doi: 10.1128/jb.173.7.2265-2270.1991.
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