Urban J F, Maliszewski C R, Madden K B, Katona I M, Finkelman F D
Helminthic Disease Laboratory, U.S. Department of Agriculture, Beltsville, MD 20705, USA.
J Immunol. 1995 May 1;154(9):4675-84.
We examined the ability of a long acting formulation of IL-4 (IL-4/anti-IL-4 mAb complexes) to limit established infections of normal and immune deficient mice with two nematode parasites: Heligmosomoides polygyrus and Nippostrongylus brasiliensis. IL-4, at a dose of 5 to 20 micrograms every 3 to 4 days, rapidly decreased egg production and, over a period of 6 to 9 days, terminated infection in H. polygyrus-inoculated BALB/c mice. IL-4 treatment also considerably decreased egg production in H. polygyrus-inoculated CB.17 severe combined immunodeficient (SCID) mice and terminated infection in N. brasiliensis-inoculated SCID mice and anti-CD4 mAb-treated BALB/c mice. IL-4 was less effective at limiting H. polygyrus infection if administrated when parasites were in larval stages than if administered after adult worms had developed. The effects of IL-4 were inhibited completely by an mAb that specifically blocks the mouse IL-4R. These observations demonstrate that IL-4 can limit the fecundity and survival of gastrointestinal nematode parasites through effects on the host that are independent of the specific immune system.
我们研究了长效白细胞介素4制剂（IL-4/抗IL-4单克隆抗体复合物）限制正常小鼠和免疫缺陷小鼠感染两种线虫寄生虫（多房棘球绦虫和巴西日圆线虫）的能力。每3至4天给予5至20微克剂量的IL-4，可迅速减少多房棘球绦虫接种的BALB/c小鼠的产卵量，并在6至9天内终止感染。IL-4治疗还显著降低了多房棘球绦虫接种的CB.17严重联合免疫缺陷（SCID）小鼠的产卵量，并终止了巴西日圆线虫接种的SCID小鼠和抗CD4单克隆抗体处理的BALB/c小鼠的感染。如果在寄生虫处于幼虫阶段时给予IL-4，其限制多房棘球绦虫感染的效果不如在成虫发育后给予。特异性阻断小鼠IL-4受体的单克隆抗体可完全抑制IL-4的作用。这些观察结果表明，IL-4可通过对宿主的作用来限制胃肠道线虫寄生虫的繁殖力和存活率，而这种作用独立于特异性免疫系统。
