Department of Immunology, Theodor Bilharz Research Institute, Cairo, Egypt.
Department of Parasitology, Theodor Bilharz Research Institute, Cairo, Egypt.
BMC Infect Dis. 2024 Nov 28;24(1):1360. doi: 10.1186/s12879-024-10202-9.
Trichinellosis is a zoonotic, foodborne parasitic infection causing muscle damage. This study investigated the potential therapeutic effects of the commercially available probiotic treatment Linex, both alone and in combination with Albendazole (ALB), on the intestinal and muscular stages of Trichinella spiralis infection in mice, assessing outcomes through parasitological, immunological, and histopathological measures. This study is the first to demonstrate the synergistic effect of combining the commercially available probiotic Linex with Albendazole for trichinellosis treatment. By enhancing both parasitological and immunological outcomes, this combined therapy not only significantly reduces parasite burden but also modulates the immune response, shifting it toward a protective Th1 profile. In parasitological terms, the highest adult and larval count reduction was observed in combined Linex and Albendazole treatment (100%, 97.7%) respectively. Lesser percentage of reduction were recorded in Linex alone therapy (43.2%, 88.4%) respectively. Histopathologically there was amelioration of the inflammatory cellular infiltration in all treated groups with best results in combined Linex and Albendazole treatment. Immunologically, serum IFN-γ levels increased significantly in all treated groups with highest levels in combined Linex and Albendazole treatment, while IL-4 and IL-13 level decreased significantly in all treated groups with best results observed in Linex alone treatment. To conclude; combined Linex and Albendazole treatment of mice infected with T. spirals could ameliorate the infection and improve the immune response.
旋毛虫病是一种人畜共患的食源性寄生虫感染，可导致肌肉损伤。本研究调查了市售益生菌治疗药物利奈克斯（Linex）单独使用和与阿苯达唑（ALB）联合使用对感染旋毛虫的小鼠肠道和肌肉阶段的潜在治疗效果，通过寄生虫学、免疫学和组织病理学措施评估结果。本研究首次证明了将市售益生菌 Linex 与阿苯达唑联合用于旋毛虫病治疗的协同作用。通过增强寄生虫学和免疫学结果，这种联合疗法不仅显著降低寄生虫负荷，而且还调节免疫反应，使其向保护性 Th1 表型转变。在寄生虫学方面，联合使用 Linex 和 Albendazole 的成虫和幼虫计数减少率最高（分别为 100%和 97.7%）。单独使用 Linex 的治疗的减少率较低（分别为 43.2%和 88.4%）。组织病理学上，所有治疗组的炎症细胞浸润均有所改善，联合使用 Linex 和 Albendazole 的治疗效果最佳。免疫学方面，所有治疗组的血清 IFN-γ 水平均显著升高，联合使用 Linex 和 Albendazole 的治疗组水平最高，而所有治疗组的 IL-4 和 IL-13 水平均显著降低，单独使用 Linex 的治疗组效果最佳。总之，联合使用 Linex 和 Albendazole 治疗感染旋毛虫的小鼠可以改善感染并改善免疫反应。
