Suzuki M, Hayashi Y
Jpn J Microbiol. 1975 Oct;19(5):355-62. doi: 10.1111/j.1348-0421.1975.tb00892.x.
Guinea pigs sensitized with purified galactomannan from Aspergillus fumigatus and mannan from Candida albicans, each containing negligible quantities of nitrogen, were examined for their immunological responses against the corresponding polysaccharides with respect to the delayed-type skin reaction and the macrophage migration inhibition phenomenon. In both cases, the delayed-type skin reaction test and the macrophage migration inhibition test showed positive results. The reactivity was stronger in animals sensitized with polysaccharides in Freund's complete adjuvant than those sensitized with the same polysaccharides in Freund's incomplete adjuvant. Polysaccharides chemically modified by partial acid degradation or by periodate oxidation were found to be completely incapable of eliciting such immune responses. These results are also discussed in relation to the antigenic determinant of the polysaccharides in such immune responses and the precipitin reaction previously observed by us and other investigators.
用来自烟曲霉的纯化半乳甘露聚糖和来自白色念珠菌的甘露聚糖（每种含可忽略不计的氮量）致敏的豚鼠，针对相应多糖的迟发型皮肤反应和巨噬细胞游走抑制现象检测其免疫反应。在这两种情况下，迟发型皮肤反应试验和巨噬细胞游走抑制试验均呈阳性结果。用弗氏完全佐剂中的多糖致敏的动物比用弗氏不完全佐剂中的相同多糖致敏的动物反应性更强。发现经部分酸降解或高碘酸盐氧化化学修饰的多糖完全不能引发此类免疫反应。还结合此类免疫反应中多糖的抗原决定簇以及我们和其他研究者先前观察到的沉淀素反应对这些结果进行了讨论。
