Bailey D N
Department of Pathology, University of California Medical Center, San Diego 92103-8320.
J Anal Toxicol. 1995 Jan-Feb;19(1):1-4. doi: 10.1093/jat/19.1.1.
Formation of cocapropylene (CP), the n-propyl analogue of cocaine, by human liver has been demonstrated for the first time through incubation of whole liver homogenates with cocaine (COC) and n-propanol (PrOH). The formation of CP was noted by 30 min in each case, and it continued throughout the 6-h study period. The concentration of COC declined throughout each incubation. Sodium fluoride was found to inhibit completely the formation of CP. For control purposes, the capability of each liver to generate cocaethylene (CE), the known transesterification product of COC and ethanol (EtOH), was established by separate incubation of the homogenate with COC and EtOH. Concentrations of COC, CP, and CE in the respective homogenates were measured by reversed-phase high-performance liquid chromatography, whereas those of EtOH and PrOH were measured by gas-liquid chromatography.
通过将全肝匀浆与可卡因（COC）和正丙醇（PrOH）一起孵育，首次证明了人体肝脏能够形成可卡因的正丙基类似物——共丙基可卡因（CP）。在每种情况下，30分钟时就注意到了CP的形成，并且在整个6小时的研究期间持续存在。在每次孵育过程中，COC的浓度都在下降。发现氟化钠能完全抑制CP的形成。为了进行对照，通过将匀浆与COC和乙醇（EtOH）单独孵育，确定了每个肝脏产生古柯乙烯（CE）的能力，CE是COC和EtOH已知的酯交换产物。通过反相高效液相色谱法测量各自匀浆中COC、CP和CE的浓度，而通过气液色谱法测量EtOH和PrOH的浓度。
