衰老雌性小鼠大脑中胶质纤维酸性蛋白的增加。

Increases of glial fibrillary acidic protein in the aging female mouse brain.

作者信息

Kohama S G, Goss J R, Finch C E, McNeill T H

机构信息

University of Southern California, Andrus Gerontology Center, Los Angeles 90089-0191, USA.

出版信息

Neurobiol Aging. 1995 Jan-Feb;16(1):59-67. doi: 10.1016/0197-4580(95)80008-f.

DOI:10.1016/0197-4580(95)80008-f

PMID:7723937

Abstract

Age-related increases of the astrocyte marker, glial fibrillary acidic protein (GFAP), were further resolved by in situ hybridization and immunocytochemistry in female C57BL/6J mice. The age groups represented the major stages of reproductive aging: young (5 months), middle-age (18 months), and old (23 and 26 months). GFAP mRNA and protein showed generalized increases in old mice. Major white fiber tracts, such as the corpus callosum, fimbria, stria terminalis, and optic tract, showed increased GFAP immunostaining and mRNA. Gray matter showed robust > or = twofold increases in GFAP mRNA with age, especially in the thalamus and hypothalamus, areas that expressed little GFAP in the young. These generalized age-related increases of GFAP in many brain regions imply the existence of a widespread stimulus for increased activity of astrocytes during aging.

摘要

通过原位杂交和免疫细胞化学技术，在雌性C57BL/6J小鼠中进一步解析了与年龄相关的星形胶质细胞标志物——胶质纤维酸性蛋白（GFAP）的增加情况。这些年龄组代表了生殖衰老的主要阶段：年轻（5个月）、中年（18个月）和老年（23和26个月）。GFAP mRNA和蛋白在老年小鼠中普遍增加。主要的白质纤维束，如胼胝体、穹窿、终纹和视束，显示出GFAP免疫染色和mRNA增加。灰质中GFAP mRNA随年龄增长呈现强劲的≥两倍增加，特别是在丘脑和下丘脑，这些区域在年轻时GFAP表达很少。许多脑区中这些与年龄相关的GFAP普遍增加意味着在衰老过程中存在广泛刺激星形胶质细胞活性增加的因素。