Hwang D F, Cheng C A, Jeng S S
Department of Marine Food Science, National Taiwan Ocean University, Keelung, Republic of China.
Toxicon. 1994 Dec;32(12):1573-9. doi: 10.1016/0041-0101(94)90316-6.
Paralytic toxicity was detected in the gastropod mollusc Niotha clathrata collected from South Taiwan in April and November 1993. Each seasonal toxin was partially purified from toxic specimens of N. clathrata by ultrafiltration using a membrane (Diaflo YM-2), followed by chromatography on a column (Bio-Gel P-2). Two toxin fractions (I and II) were then obtained for each seasonal shell toxin. The ratio of fraction I to fraction II for each seasonal shell toxin was about 4:1 according to tetrodotoxin bioassay. Based on analyses by TLC, electrophoresis, and HPLC, fraction I toxin contained tetrodotoxin and its derivative anhydrotetrodotoxin, and fraction II toxin contained gonyautoxin-3 for each seasonal shell toxin.
1993年4月和11月从台湾南部采集的腹足纲软体动物方格织纹螺中检测到麻痹性毒素。通过使用膜（Diaflo YM - 2）超滤，然后在柱（Bio - Gel P - 2）上进行色谱分离，从方格织纹螺的有毒标本中对每种季节性毒素进行了部分纯化。然后针对每种季节性贝壳毒素获得了两个毒素组分（I和II）。根据河豚毒素生物测定，每种季节性贝壳毒素的组分I与组分II的比例约为4:1。基于薄层层析（TLC）、电泳和高效液相色谱（HPLC）分析，对于每种季节性贝壳毒素，组分I毒素含有河豚毒素及其衍生物脱水河豚毒素，组分II毒素含有石房蛤毒素 - 3。
