台湾两种扇蟹（花纹爱洁蟹和雷诺氏怪蟹）的毒性及有毒成分

Toxicity and toxic components of two xanthid crabs, Atergatis floridus and Demania reynaudi, in Taiwan.

作者信息

Tsai Y H, Hwang D F, Chai T J, Jeng S S

机构信息

Department of Marine Food Science, National Taiwan Ocean University, Keelung, R.O.C.

出版信息

Toxicon. 1997 Aug;35(8):1327-35. doi: 10.1016/s0041-0101(97)00005-6.

DOI:10.1016/s0041-0101(97)00005-6

PMID:9278980

Abstract

Paralytic toxicity was detected by tetrodotoxin bioassay in eight specimens of Atergatis floridus and seven specimens of Demania reynaudi, collected from Taiwan in 1994. The toxicity of crab specimens was 161 +/- 115 (mean +/- S.D.) mouse units (MU) for A. floridus and 640 +/- 273 MU for D. reynaudi. The respective toxins were partially purified from specimens of A. floridus and D. reynaudi by ultrafiltration using a Diaflo YM-1 membrane, followed by chromatography on a Bio-Gel P-2 column. Electrophoresis, thin-layer chromatography, high-performance liquid chromatography, ultraviolet spectrum and gas chromatography-mass spectrometry indicated that the toxin of A. floridus was mainly composed of tetrodotoxin (85%), along with minor gonyautoxin 1-4 (15%), and the toxin of D. reynaudi was mainly composed of tetrodotoxin (88%), along with minor gonyautoxin 2-4 and neosaxitoxin (12%).

摘要

1994年从台湾采集的8个花纹爱洁蟹标本和7个雷诺氏蝉蟹标本，通过河豚毒素生物测定法检测到麻痹毒性。花纹爱洁蟹标本的毒性为161±115（平均值±标准差）小鼠单位（MU），雷诺氏蝉蟹为640±273 MU。分别使用Diaflo YM - 1膜通过超滤从花纹爱洁蟹和雷诺氏蝉蟹标本中部分纯化毒素，然后在Bio - Gel P - 2柱上进行色谱分析。电泳、薄层色谱、高效液相色谱、紫外光谱和气相色谱 - 质谱分析表明，花纹爱洁蟹的毒素主要由河豚毒素（85%）组成，还有少量的膝沟藻毒素1 - 4（15%），雷诺氏蝉蟹的毒素主要由河豚毒素（88%）组成，还有少量的膝沟藻毒素2 - 4和新石房蛤毒素（12%）。