台湾螃蟹Atergatopsis germaini中麻痹性毒素的存在情况。

Occurrence of paralytic toxin in Taiwanese crab Atergatopsis germaini.

作者信息

Tsai Y H, Hwang D F, Chai T J, Jeng S S

机构信息

Department of Marine Food Science, National Taiwan Ocean University, Keelung, Republic of China.

出版信息

Toxicon. 1996 Apr;34(4):467-74. doi: 10.1016/0041-0101(95)00149-2.

DOI:10.1016/0041-0101(95)00149-2

PMID:8735246

Abstract

Paralytic toxicity was detected by paralytic shellfish poison bioassay for all 17 specimens of the xanthid crab A. germaini collected from northern Taiwan in November 1993. The average toxicity of crab specimens was 3809 +/- 2591 mouse units (mean +/- S.D.). The toxin was partially purified from ethanolic extract of the crab by ultrafiltration and Bio-Gel P-2 column chromatography. Electrophoresis, TLC, HPLC, ultraviolet spectrum and GC-MS analyses indicated that the crab toxin was composed of gonyautoxin 3 (50%), neosaxitoxin and saxitoxin (7%), a novel paralytic shellfish poison-like toxin (40%) and tetrodotoxin (3%).

摘要

1993年11月从台湾北部采集的17只杰氏黄道蟹标本，经麻痹性贝类毒素生物测定均检测到麻痹性毒性。蟹标本的平均毒性为3809±2591鼠单位（平均值±标准差）。通过超滤和Bio-Gel P-2柱色谱法从蟹的乙醇提取物中部分纯化毒素。电泳、薄层色谱、高效液相色谱、紫外光谱和气相色谱-质谱分析表明，蟹毒素由3型膝沟藻毒素（50%）、新石房蛤毒素和石房蛤毒素（7%）、一种新型麻痹性贝类毒素样毒素（40%）和河豚毒素（3%）组成。