Sallander S, Shanwell A, Pegert S, Jakobsson L, Wiechel B
Stockholm Blood Transfusion Service, Södersjukhuset, Sweden.
Vox Sang. 1995;68(1):22-6. doi: 10.1111/j.1423-0410.1995.tb02540.x.
We have modified previously described solid-phase tests for erythrocyte antibody screening to develop a method, suitable for antiglobulin- and enzyme-enhanced techniques (SPH-IAT and SPH-ENZ). In this study we compared the SPH tests with an autoanalyzer (AA) technique. The results were more specific with the SPH tests than with the AA. Of 4,234 unselected samples from pregnant women, screen-positive samples were reduced from 96 (2.27%) by the AA, to 56 (1.32%) by the SPH tests. This difference was due to the reduced number of false-positive reactions with the SPH tests, 0.47% compared to 1.44% with the AA. Antibodies detected by the AA and the SPH-IAT and -ENZ were: 9 Rh prophylaxis, 2 anti-c, 1 anti-K and anti-M, and 1 anti-Jka. Antibodies detected only by the SPH tests were 1 anti-K, 1 anti-Le(a) (SPH-IAT and -ENZ), 1 anti-M (SPH-IAT) and 4 anti-Jka (SPH-ENZ). One anti-C, 2 anti-D, 3 Rh prophylaxis and 1 anti-E were detected by the AA and the SPH-ENZ but failed to react by the SPH-IAT. One anti-Le(a) and 8 Rh prophylaxis antibodies were detected by AA only. All clinically important antibodies were detected by the SPH tests. We conclude that the SPH-IAT and SPH-ENZ are screening methods with high specificity that are readily adaptable to larger series of samples from pregnant women and suitable for automated handling throughout the screening and identification process.
我们改进了先前描述的用于红细胞抗体筛查的固相试验，以开发一种适用于抗球蛋白和酶增强技术（固相抗球蛋白试验和固相酶试验）的方法。在本研究中，我们将固相试验与自动分析仪技术进行了比较。固相试验的结果比自动分析仪技术更具特异性。在4234份未筛选的孕妇样本中，自动分析仪检测出的筛查阳性样本为96份（2.27%），而固相试验检测出的为56份（1.32%）。这种差异是由于固相试验的假阳性反应数量减少，固相试验为0.47%，而自动分析仪为1.44%。自动分析仪、固相抗球蛋白试验和固相酶试验检测出的抗体有：9份Rh预防抗体、2份抗 - c、1份抗 - K、1份抗 - M和1份抗 - Jka。仅通过固相试验检测出的抗体有1份抗 - K、1份抗 - Le(a)（固相抗球蛋白试验和固相酶试验）、1份抗 - M（固相抗球蛋白试验）和4份抗 - Jka（固相酶试验）。自动分析仪和固相酶试验检测出1份抗 - C、2份抗 - D、3份Rh预防抗体和1份抗 - E，但固相抗球蛋白试验未反应。仅自动分析仪检测出1份抗 - Le(a)和8份Rh预防抗体。所有具有临床意义的抗体均通过固相试验检测出。我们得出结论，固相抗球蛋白试验和固相酶试验是特异性高的筛查方法，易于适用于更多孕妇样本系列，并且适用于整个筛查和鉴定过程的自动化处理。
