Michalewska B, Walewska I, Seyfriedowa H, Kuśnierz-Alejska G
Zakładu Serologii Instytutu Hematologii, Warszawie.
Acta Haematol Pol. 1991;22(1):105-12.
A series of comparative investigations was carried out for establishing the value of the indirect antiglobulin test (IAT) in its first phase. The results showed that PEG, in relation to NaCl solution of low ionic strength, increased the reactivity of various antibodies of different specificity present in 72 studied sera. A significant superiority of IAT-PEG to IAT-BISS was noted in 45.8% of these sera. In search for antibodies in sera of 1160 patients IAT-PEG detected 7 antibodies not reacting in the IAT-LISS, and 5 of them had Rh system specificity, one was anti-K and one anti-Jka. Although the IAT-PEG was more sensitive than IAT-LISS in the detection of anti-Rh antibodies, it was less sensitive in this range than the enzymatic LEN test with which not only 5 of these antibodies were demonstrated but which detected also 8 antibodies not demonstrated in IAT-PEG or IAT-LISS. All 5 antibodies not detected by the IAT-PEG but demonstrated in the IAT-LISS were specific for the Lewis system. The per cent of reactions regarded as non-specific in the IAT-PEG was 1.3%. The IAT-PEG modification may be recommended as a sensitive, simple and specific method extending the possibilities of immunohematological diagnosis, especially in cases of post-transfusion reactions and in difficulties of interpretation connected with the occurrence of weakly positive reactions in the IAT-LISS.
为确定间接抗球蛋白试验（IAT）第一阶段的价值，进行了一系列对比研究。结果表明，与低离子强度的NaCl溶液相比，聚乙二醇（PEG）可增强72份研究血清中存在的各种不同特异性抗体的反应性。在这些血清中，45.8%的血清显示IAT-PEG显著优于IAT-BISS。在检测1160例患者血清中的抗体时，IAT-PEG检测出7种在IAT-LISS中无反应的抗体，其中5种具有Rh系统特异性，1种为抗-K，1种为抗-Jka。虽然IAT-PEG在检测抗Rh抗体方面比IAT-LISS更敏感，但在该范围内它比酶联免疫吸附试验（LEN试验）敏感性低，LEN试验不仅能检测出其中5种抗体，还能检测出8种在IAT-PEG或IAT-LISS中未检测到的抗体。IAT-PEG未检测到但在IAT-LISS中检测出的所有5种抗体均为Lewis系统特异性抗体。IAT-PEG中被视为非特异性反应的比例为1.3%。IAT-PEG改良法可作为一种敏感、简单且特异的方法推荐，它扩展了免疫血液学诊断的可能性，尤其是在输血后反应以及与IAT-LISS中弱阳性反应出现相关的解释困难的情况下。
