Lagrange P
Pathol Biol (Paris). 1976 Jan;24(1):67-74.
Delayed-type hypersensitivity (DTH) develops in the absence of an adjuvant when mice are injected with an apropriate dose of Sheep Red Blood Cells (SRBC). Increasing the dose of SRBC reduces and eventually abolished all evidence of DTH and no further sensitisation can be achieved even with the optimal subcutaneously injected dose, except in splenectomised mice in whom the development of DTH is not suppressed, even by massive doses of SRBC. This is also the case in mice treated with cyclophosphamide (CY) which suppresses selectively the antibody response. Hence the suppression of T cell activity measured as DTH cannot be due to antigen as such. The serum of blocked animals partially inhibits the induction and expression of DTH, and its blocking activity increase substantially after partial absorption. Absorbed serum did not inhibit in vivo the proliferative response nor the number of plaque forming cells to SRBC in peripheral lymph nodes, it increases the hemagglutinating titer of circulating specific antibody even though DTH is totally suppressed. It seems that the products of the interaction between antigens and antibodies block the activated T cells which mediate DTH without interfering with helper cells.
当给小鼠注射适当剂量的绵羊红细胞（SRBC）时，在没有佐剂的情况下会发生迟发型超敏反应（DTH）。增加SRBC的剂量会降低并最终消除所有DTH的证据，即使采用最佳皮下注射剂量，也无法实现进一步的致敏，除非是脾切除的小鼠，在这类小鼠中，即使注射大量SRBC，DTH的发展也不会受到抑制。在用环磷酰胺（CY）治疗的小鼠中也是如此，环磷酰胺会选择性地抑制抗体反应。因此，以DTH衡量的T细胞活性的抑制不能归因于抗原本身。被阻断动物的血清会部分抑制DTH的诱导和表达，并且在部分吸收后其阻断活性会大幅增加。吸收后的血清在体内既不抑制增殖反应，也不抑制外周淋巴结中针对SRBC的噬斑形成细胞数量，它会增加循环特异性抗体的血凝滴度，即使DTH被完全抑制。似乎抗原与抗体相互作用的产物会阻断介导DTH的活化T细胞，而不会干扰辅助细胞。
