Mackaness G B, Lagrange P H, Miller T E, Ishibashi T
J Exp Med. 1974 Mar 1;139(3):543-59. doi: 10.1084/jem.139.3.543.
An explanation was sought for the fact that delayed-type hypersensitivity (DTH) does not normally occur in response to T-cell-dependent antigens unless an adjuvant is used. But when sheep red blood cells (SRBC) were administered intravenously DTH did appear, provided that the dose of antigen was less than that required to give a maximum antibody response. Animals in which T-cell activity had been blocked by a large dose of antigen could not be sensitized adoptively, and their spleen cells failed to transfer DTH to normal recipients. The serum of blocked animals partially inhibited the induction of DTH, and after absorption with SRBC its blocking activity increased substantially. Moreover, absorbed serum inhibited DTH in previously sensitized animals, but it did not inhibit the proliferative response to SRBC in peripheral lymph nodes or reduce the number of plaque-forming cells produced therein. On the contrary, the hemagglutinating titer was actually increased by blocking serum even though DTH was totally suppressed. It is concluded that a product of the interaction between antigens and antibody blocks the activated T cells which mediate DTH without interfering with helper cells.
对于迟发型超敏反应（DTH）通常不会在没有使用佐剂的情况下对T细胞依赖性抗原产生反应这一事实，人们一直在寻求解释。但是，当静脉注射绵羊红细胞（SRBC）时，只要抗原剂量小于产生最大抗体反应所需的剂量，DTH就会出现。用大剂量抗原阻断T细胞活性的动物不能被过继致敏，并且它们的脾细胞不能将DTH转移给正常受体。被阻断动物的血清部分抑制了DTH的诱导，在用SRBC吸收后，其阻断活性大幅增加。此外，吸收后的血清抑制了先前致敏动物的DTH，但它没有抑制外周淋巴结对SRBC的增殖反应，也没有减少其中产生的噬斑形成细胞的数量。相反，尽管DTH被完全抑制，但阻断血清实际上提高了血凝滴度。得出的结论是，抗原与抗体相互作用的产物阻断了介导DTH的活化T细胞，而不干扰辅助细胞。
