白色念珠菌中的多重耐药性：BENr基因的破坏
Multidrug resistance in Candida albicans: disruption of the BENr gene.
作者信息
Goldway M, Teff D, Schmidt R, Oppenheim A B, Koltin Y
机构信息
Department of Molecular Genetics, Hebrew University Hadassah Medical School, Jerusalem, Israel.
出版信息
Antimicrob Agents Chemother. 1995 Feb;39(2):422-6. doi: 10.1128/AAC.39.2.422.
Abstract
The BENr gene of Candida albicans, which confers resistance on susceptible strains of Saccharomyces cerevisiae to six structurally and functionally unrelated drugs, was described recently (R. Ben-Yaacov, S. Knoller, G. Caldwell, J. M. Becker, and Y. Koltin, Antimicrob. Agents Chemother. 38:648-652, 1994). This gene bears similarity to membrane proteins encoding antibiotic resistance in prokaryotes and eukaryotes. The effect of disruption of this gene on viability and drug susceptibility was determined. The results indicate that the gene is not essential but its inactivation leads to susceptibility to three of the four drugs tested. Inactivation of this gene did not increase the susceptibility of the mutant to benomyl, suggesting that C. albicans has other mechanisms of resistance, some of which may be additional efflux pumps that confer resistance to this tubulin-destabilizing agent.
摘要
白色念珠菌的BENr基因最近被报道，该基因可使酿酒酵母的敏感菌株对六种结构和功能不相关的药物产生抗性（R. Ben-Yaacov、S. Knoller、G. Caldwell、J. M. Becker和Y. Koltin，《抗菌剂与化疗》38:648 - 652，1994年）。该基因与原核生物和真核生物中编码抗生素抗性的膜蛋白具有相似性。确定了该基因缺失对生存能力和药物敏感性的影响。结果表明该基因并非必需基因，但其失活会导致对所测试的四种药物中的三种产生敏感性。该基因的失活并未增加突变体对苯菌灵的敏感性，这表明白色念珠菌具有其他抗性机制，其中一些可能是赋予对这种微管蛋白不稳定剂抗性的额外外排泵。
相似文献
1
Multidrug resistance in Candida albicans: disruption of the BENr gene.白色念珠菌中的多重耐药性：BENr基因的破坏
Antimicrob Agents Chemother. 1995 Feb;39(2):422-6. doi: 10.1128/AAC.39.2.422.
2
Candida albicans gene encoding resistance to benomyl and methotrexate is a multidrug resistance gene.编码对苯菌灵和甲氨蝶呤耐药性的白色念珠菌基因是一种多药耐药基因。
Antimicrob Agents Chemother. 1994 Apr;38(4):648-52. doi: 10.1128/AAC.38.4.648.
3
Mechanisms of resistance to azole antifungal agents in Candida albicans isolates from AIDS patients involve specific multidrug transporters.从艾滋病患者分离出的白色念珠菌中，对唑类抗真菌药物产生耐药性的机制涉及特定的多药转运蛋白。
Antimicrob Agents Chemother. 1995 Nov;39(11):2378-86. doi: 10.1128/AAC.39.11.2378.
4
A novel multidrug efflux transporter gene of the major facilitator superfamily from Candida albicans (FLU1) conferring resistance to fluconazole.一种来自白色念珠菌的主要易化子超家族的新型多药外排转运体基因（FLU1），赋予对氟康唑的抗性。
Microbiology (Reading). 2000 Nov;146 ( Pt 11):2743-2754. doi: 10.1099/00221287-146-11-2743.
5
Susceptibilities of Candida albicans multidrug transporter mutants to various antifungal agents and other metabolic inhibitors.白色念珠菌多药转运蛋白突变体对各种抗真菌剂和其他代谢抑制剂的敏感性。
Antimicrob Agents Chemother. 1996 Oct;40(10):2300-5. doi: 10.1128/AAC.40.10.2300.
6
Analysis of a Candida albicans gene that encodes a novel mechanism for resistance to benomyl and methotrexate.对白色念珠菌中一个编码对苯菌灵和甲氨蝶呤抗性新机制的基因的分析。
Mol Gen Genet. 1991 Jun;227(2):318-29. doi: 10.1007/BF00259685.
7
Isolation of a putative Candida albicans transcriptional regulator involved in pleiotropic drug resistance by functional complementation of a pdr1 pdr3 mutation in Saccharomyces cerevisiae.通过对酿酒酵母中pdr1 pdr3突变进行功能互补来分离一种推定的参与多药耐药性的白色念珠菌转录调节因子。
J Bacteriol. 1999 Jan;181(1):231-40. doi: 10.1128/JB.181.1.231-240.1999.
8
Functional expression of the Candida albicans beta-tubulin gene in Saccharomyces cerevisiae.白色念珠菌β-微管蛋白基因在酿酒酵母中的功能表达。
Gene. 1990 May 31;90(1):115-23. doi: 10.1016/0378-1119(90)90446-x.
9
MDR1-mediated drug resistance in Candida dubliniensis.都柏林念珠菌中多药耐药蛋白1介导的耐药性
Antimicrob Agents Chemother. 2001 Dec;45(12):3416-21. doi: 10.1128/AAC.45.12.3416-3421.2001.
10
Divergent functions of three Candida albicans zinc-cluster transcription factors (CTA4, ASG1 and CTF1) complementing pleiotropic drug resistance in Saccharomyces cerevisiae.三种白色念珠菌锌簇转录因子（CTA4、ASG1和CTF1）在酿酒酵母中互补多药耐药性的不同功能
Microbiology (Reading). 2008 May;154(Pt 5):1491-1501. doi: 10.1099/mic.0.2007/016063-0.
引用本文的文献
1
Celastrol boosts fluconazole efficacy against vaginal candidiasis: in vitro and in vivo evidence.雷公藤红素增强氟康唑对阴道念珠菌病的疗效：体外和体内证据
AMB Express. 2025 Jan 29;15(1):18. doi: 10.1186/s13568-025-01824-6.
2
The anti-staphylococcal fusidic acid as an efflux pump inhibitor combined with fluconazole against vaginal candidiasis in mouse model.抗葡萄球菌的夫西地酸作为外排泵抑制剂与氟康唑联合治疗阴道念珠菌病的小鼠模型。
BMC Microbiol. 2024 Feb 10;24(1):54. doi: 10.1186/s12866-024-03181-z.
3
Directed Mutational Strategies Reveal Drug Binding and Transport by the MDR Transporters of .定向突变策略揭示了……的多药耐药转运蛋白的药物结合与转运情况 。 （原文句子不完整，翻译可能不太准确，你可补充完整原文再次提问。）
J Fungi (Basel). 2021 Jan 20;7(2):68. doi: 10.3390/jof7020068.
4
An Azole-Tolerant Endosomal Trafficking Mutant of Candida albicans Is Susceptible to Azole Treatment in a Mouse Model of Vaginal Candidiasis.白色念珠菌的一种耐唑类内体运输突变体在阴道念珠菌病小鼠模型中对唑类治疗敏感。
Antimicrob Agents Chemother. 2017 May 24;61(6). doi: 10.1128/AAC.00084-17. Print 2017 Jun.
5
Endosomal Trafficking Defects Can Induce Calcium-Dependent Azole Tolerance in Candida albicans.内体运输缺陷可诱导白色念珠菌产生钙依赖性唑耐受性。
Antimicrob Agents Chemother. 2016 Nov 21;60(12):7170-7177. doi: 10.1128/AAC.01034-16. Print 2016 Dec.
6
Mutational Analysis of Intracellular Loops Identify Cross Talk with Nucleotide Binding Domains of Yeast ABC Transporter Cdr1p.细胞内环的突变分析揭示了与酵母ABC转运蛋白Cdr1p核苷酸结合结构域的相互作用。
Sci Rep. 2015 Jun 8;5:11211. doi: 10.1038/srep11211.
7
Trafficking through the late endosome significantly impacts Candida albicans tolerance of the azole antifungals.通过晚期内体的运输显著影响白色念珠菌对唑类抗真菌药物的耐受性。
Antimicrob Agents Chemother. 2015 Apr;59(4):2410-20. doi: 10.1128/AAC.04239-14. Epub 2015 Feb 9.
8
MFS multidrug transporters in pathogenic fungi: do they have real clinical impact?致病真菌中的MFS多药转运蛋白：它们真的有临床影响吗？
Front Physiol. 2014 May 28;5:197. doi: 10.3389/fphys.2014.00197. eCollection 2014.
9
Insight into pleiotropic drug resistance ATP-binding cassette pump drug transport through mutagenesis of Cdr1p transmembrane domains.通过突变 Cdr1p 跨膜结构域深入了解多药耐药性 ABC 泵药物转运。
J Biol Chem. 2013 Aug 23;288(34):24480-93. doi: 10.1074/jbc.M113.488353. Epub 2013 Jul 3.
10
The dual role of candida glabrata drug:H+ antiporter CgAqr1 (ORF CAGL0J09944g) in antifungal drug and acetic acid resistance.光滑假丝酵母药物:H+ 反向转运蛋白 CgAqr1（ORF CAGL0J09944g）在抗真菌药物和乙酸抗性中的双重作用。
Front Microbiol. 2013 Jun 26;4:170. doi: 10.3389/fmicb.2013.00170. eCollection 2013.
本文引用的文献
1
Resistance of yeasts to azole-derivative antifungals.酵母对唑类衍生物抗真菌药物的耐药性。
J Antimicrob Chemother. 1993 Apr;31(4):463-71. doi: 10.1093/jac/31.4.463.
2
Natural cycloheximide resistance in yeast. The role of ribosomal protein L41.酵母中天然的放线菌酮抗性。核糖体蛋白L41的作用。
Eur J Biochem. 1993 Apr 15;213(2):841-8. doi: 10.1111/j.1432-1033.1993.tb17827.x.
3
Topology, structure and evolution of two families of proteins involved in antibiotic and antiseptic resistance in eukaryotes and prokaryotes--an analysis.真核生物和原核生物中涉及抗生素和防腐剂抗性的两类蛋白质的拓扑结构、结构与进化——一项分析
Gene. 1993 Feb 14;124(1):1-11. doi: 10.1016/0378-1119(93)90755-r.
4
Isogenic strain construction and gene mapping in Candida albicans.白色念珠菌的同基因菌株构建与基因定位
Genetics. 1993 Jul;134(3):717-28. doi: 10.1093/genetics/134.3.717.
5
The amiloride resistance gene, car1, of Schizosaccharomyces pombe.粟酒裂殖酵母的阿米洛利抗性基因car1。
Mol Gen Genet. 1993 Nov;241(3-4):298-304. doi: 10.1007/BF00284681.
6
The ARG4 gene of Candida albicans.白色念珠菌的ARG4基因。
Gene. 1994 May 16;142(2):213-8. doi: 10.1016/0378-1119(94)90263-1.
7
Genetics and biochemistry of yeast multidrug resistance.酵母多药耐药性的遗传学与生物化学
Biochim Biophys Acta. 1994 Aug 30;1187(2):152-62. doi: 10.1016/0005-2728(94)90102-3.
8
Candida albicans gene encoding resistance to benomyl and methotrexate is a multidrug resistance gene.编码对苯菌灵和甲氨蝶呤耐药性的白色念珠菌基因是一种多药耐药基因。
Antimicrob Agents Chemother. 1994 Apr;38(4):648-52. doi: 10.1128/AAC.38.4.648.
9
A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance.酵母中缺乏乳清苷-5'-磷酸脱羧酶活性的突变体的正向选择：5-氟乳清酸抗性。
Mol Gen Genet. 1984;197(2):345-6. doi: 10.1007/BF00330984.
10
Transformation of intact yeast cells treated with alkali cations.经碱金属阳离子处理的完整酵母细胞的转化
J Bacteriol. 1983 Jan;153(1):163-8. doi: 10.1128/jb.153.1.163-168.1983.
Zotero 插件