Boehncke W H, Dahlke A, Zollner T M, Sterry W
Department of Dermatology, University of Ulm, Germany.
Arch Dermatol Res. 1994;287(1):68-71. doi: 10.1007/BF00370721.
Autoimmunity and microbial agents have been suggested as playing a pathogenetic role in psoriasis. Since immune responses to microbial infections are often directed towards heat shock proteins (HSP), we investigated the expression of three HSP families in normal and inflamed human skin. Specimens from ten patients with psoriasis and three patients with positive patch tests for nickel and from five healthy volunteers were analysed by means of immunohistochemistry. The patterns observed were qualitatively similar in these conditions showing only minor quantitative differences. Psoriatic epidermis exhibited the highest level of expression. HSP27, HSP70 and heat shock cognate protein 70 (HSC70) were readily detectable. HSP27 was homogeneously distributed throughout the epidermis, whereas HSP70 was restricted to the basal layer and HSC70 primarily to the suprabasal layers. Other HSPs were detected to a lesser degree and showed a more irregular pattern. Thus, the qualitative expression pattern of HSPs seems to be constant between different skin conditions, but the expression of constitutive and inducible HSP70 depends on the differentiation state of keratinocytes.
自身免疫和微生物因子被认为在银屑病发病机制中起作用。由于对微生物感染的免疫反应通常针对热休克蛋白（HSP），我们研究了三个HSP家族在正常和炎症性人类皮肤中的表达。通过免疫组织化学分析了10例银屑病患者、3例镍斑贴试验阳性患者和5名健康志愿者的标本。在这些情况下观察到的模式在质量上相似，仅显示出微小的数量差异。银屑病表皮表现出最高水平的表达。HSP27、HSP70和热休克同源蛋白70（HSC70）易于检测到。HSP27均匀分布于整个表皮，而HSP70局限于基底层，HSC70主要分布于基底上层。其他HSPs检测程度较低，且表现出更不规则的模式。因此，HSPs的定性表达模式在不同皮肤状况之间似乎是恒定的，但组成型和诱导型HSP70的表达取决于角质形成细胞的分化状态。
