Boehncke W H, Küenzlen C, Zollner T M, Mielke V, Sterry W
Department of Dermatology, University of Ulm, Germany.
Exp Dermatol. 1994 Aug;3(4):161-3. doi: 10.1111/j.1600-0625.1994.tb00272.x.
To investigate the role of T cells in psoriasis, we analyzed the T-cell receptor (TCR) repertoire in peripheral blood lymphocytes (PBLs) and in skin lesions of 9 patients with chronic plaque-stage psoriasis by means of immunohistochemistry. An extended panel of monoclonal antibodies detecting 11 different V beta families was employed in this study. In the peripheral blood, no predominant TCR V beta expression was detected and interindividual differences were small. Skin-infiltrating T cells showed only marginal differences in the TCR V beta usage when compared with PBLs. However, in 3 patients the intraepidermal infiltrate comprised up to 40% of T cells expressing a TCR V beta region which was virtually absent in the peripheral blood and the dermal infiltrate. Thus, although the TCR V beta usage of skin-infiltrating T cells showed little difference compared to PBLs, several cases exhibited a significant increase of epidermotrophic T cells using one particular V beta region.
为了研究T细胞在银屑病中的作用，我们通过免疫组织化学方法分析了9例慢性斑块状银屑病患者外周血淋巴细胞（PBL）和皮肤病变中的T细胞受体（TCR）谱。本研究使用了一组可检测11种不同Vβ家族的单克隆抗体。在外周血中，未检测到主要的TCR Vβ表达，个体间差异较小。与PBL相比，皮肤浸润性T细胞在TCR Vβ使用上仅表现出微小差异。然而，在3例患者中，表皮内浸润的T细胞中表达某一TCR Vβ区域的细胞占比高达40%，而该区域在外周血和真皮浸润中几乎不存在。因此，尽管与PBL相比，皮肤浸润性T细胞的TCR Vβ使用情况差异不大，但有几例患者使用某一特定Vβ区域的亲表皮性T细胞显著增加。
