Beattie K L, Beattie W G, Meng L, Turner S L, Coral-Vazquez R, Smith D D, McIntyre P M, Dao D D
DNA Technology Laboratory, Houston Advanced Research Center, Woodlands, TX 77381, USA.
Clin Chem. 1995 May;41(5):700-6.
Microfabricated devices containing arrays of nucleic acid hybridization sites, known as genosensors, are being developed for a variety of uses in genomic analysis. A great deal of the overall genosensor development effort involves optimization of experimental conditions in the actual use of genosensors. Here we describe a "low-tech" form of genosensor technology, involving arrays of oligonucleotides on glass microscope slides, which can be used to define optimal operating conditions and to develop applications of hybridization arrays in genome mapping and sequencing. In addition, we describe a porous silicon genosensor, which can be operated in a flowthrough mode, and discuss its advantages over current flat-surface designs. Porous silicon genosensors containing arrays of DNA fragments offer several unique capabilities in genome analysis.
包含核酸杂交位点阵列的微制造设备，即基因传感器，正被开发用于基因组分析的各种用途。基因传感器整体开发工作的很大一部分涉及在基因传感器实际使用中优化实验条件。在此，我们描述一种“低技术”形式的基因传感器技术，它涉及玻璃显微镜载玻片上的寡核苷酸阵列，可用于确定最佳操作条件，并开发杂交阵列在基因组图谱绘制和测序中的应用。此外，我们描述了一种可在流通模式下操作的多孔硅基因传感器，并讨论了其相对于当前平面设计的优势。包含DNA片段阵列的多孔硅基因传感器在基因组分析中具有多种独特功能。
