Beattie W G, Meng L, Turner S L, Varma R S, Dao D D, Beattie K L
DNA Technology Laboratory, Houston Advanced Research Center, Woodlands, TX 77381, USA.
Mol Biotechnol. 1995 Dec;4(3):213-25. doi: 10.1007/BF02779015.
Hybridization of nucleic acids to surface-tethered oligonucleotide probes has numerous potential applications in genome mapping and DNA sequence analysis. In this article, we describe a simple standard protocol for routine preparation of terminal amine-derivatized 9-mer oligonucleotide arrays on ordinary microscope slides and hybridization conditions with DNA target strands of up to several hundred bases in length with good discrimination against mismatches. Additional linker arms separating the glass surface from the probe sequence are not necessary. The technique described here offers a powerful tool for the detection of specific genetic mutations.
核酸与表面连接的寡核苷酸探针杂交在基因组作图和DNA序列分析中有许多潜在应用。在本文中，我们描述了一种简单的标准方案，用于在普通显微镜载玻片上常规制备末端胺衍生化的9聚体寡核苷酸阵列，以及与长度达数百个碱基的DNA靶链的杂交条件，对错配具有良好的区分能力。不需要额外的连接臂来分隔玻璃表面与探针序列。这里描述的技术为检测特定基因突变提供了一个强大的工具。
