Tomita T
Department of Pathology, University of Kansas Medical Center, Kansas City 66160, USA.
Dig Dis Sci. 1995 May;40(5):996-1004. doi: 10.1007/BF02064188.
To investigate the colonic adenoma-adenocarcinoma progression sequence, DNA ploidy analysis was performed on hyperplastic polyps to adenocarcinomas. DNA ploidy data were then compared with immunocytochemical staining for proliferating cell nuclear antigen (PCNA). In hyperplastic polyps to villous adenomas, all cases were diploid except one aneuploid villous adenoma. In three adenomas, diploid in situ adenocarcinomas were present. As diploid percentages decreased from hyperplastic polyps to villous adenomas, aneuploid percentages increased. In adenocarcinomas, the Dukes classification corresponded well to DNA ploidy status: all four stage A carcinomas were diploid, whereas three cases each of stage C1 and C2 carcinomas were aneuploid or multiploid. A surprising finding was that S-phase percentage in adenocarcinomas was not parallel with PCNA-positive tumor cell numbers. It is concluded that multistep adenoma-adenocarcinoma progression was partially reflected in DNA ploidy pattern from hyperplastic polyps to villous adenomas. In adenocarcinomas, the Dukes classification paralleled well the DNA ploidy status from stage A diploid to stage D aneuploid, but was not accompanied by increasing PCNA-positive cell numbers.
为了研究结肠腺瘤-腺癌的进展序列，对增生性息肉至腺癌进行了DNA倍体分析。然后将DNA倍体数据与增殖细胞核抗原（PCNA）的免疫细胞化学染色结果进行比较。在增生性息肉至绒毛状腺瘤中，除1例非整倍体绒毛状腺瘤外，所有病例均为二倍体。在3例腺瘤中，存在二倍体原位腺癌。随着从增生性息肉到绒毛状腺瘤二倍体百分比的降低，非整倍体百分比增加。在腺癌中，Dukes分期与DNA倍体状态密切相关：所有4例A期癌均为二倍体，而C1期和C2期癌各有3例为非整倍体或多倍体。一个令人惊讶的发现是，腺癌中的S期百分比与PCNA阳性肿瘤细胞数量并不平行。结论是，从增生性息肉到绒毛状腺瘤，多步骤腺瘤-腺癌进展部分反映在DNA倍体模式中。在腺癌中，Dukes分期与从A期二倍体到D期非整倍体的DNA倍体状态密切相关，但并未伴随着PCNA阳性细胞数量的增加。
