Goh H S, Jass J R
J Clin Pathol. 1986 Apr;39(4):387-92. doi: 10.1136/jcp.39.4.387.
DNA content was measured in 269 benign adenomas and 203 adenocarcinomas of the large bowel by flow cytometry. Nuclear suspensions were prepared by pepsin digestion of paraffin sections, and an adjacent section was graded histologically by one observer. Aneuploid adenomas included five of 134 (4%) showing mild dysplasia, 19 of 107 (18%) showing moderate dysplasia, and 10 of 28 (36%) showing severe dysplasia. The association between aneuploidy and grade of epithelial dysplasia was highly significant. This gives support to the adenoma (dysplasia)-carcinoma hypothesis. Carcinomas showing aneuploidy included 18 of 29 (62%) well differentiated, 92 of 144 (64%) moderately differentiated, and 19 of 30 (63%) poorly differentiated cases. There was no relation between aneuploidy and grade of carcinoma. These findings indicate an important biological difference between adenoma and carcinoma.
采用流式细胞术对269例大肠良性腺瘤和203例腺癌进行DNA含量测定。通过胃蛋白酶消化石蜡切片制备核悬液，由一名观察者对相邻切片进行组织学分级。非整倍体腺瘤中，134例轻度不典型增生者有5例（4%），107例中度不典型增生者有19例（18%），28例重度不典型增生者有10例（36%）。非整倍体与上皮不典型增生分级之间的关联极为显著。这支持了腺瘤（不典型增生）-癌假说。显示非整倍体的癌中，高分化的29例中有18例（62%），中分化的144例中有92例（64%），低分化的30例中有19例（63%）。非整倍体与癌分级之间无关联。这些发现表明腺瘤和癌之间存在重要的生物学差异。
