Garcia G M, Stalker H T, Kochert G
Department of Crop Science, North Carolina State University, Raleigh 27695-7629.
Genome. 1995 Feb;38(1):166-76. doi: 10.1139/g95-021.
Forty-six introgression lines (F10C9) from a cross between Arachis hypogaea L. (2n = 4x = 40) and A. cardenasii Krapov. & W. C. Gregory (2n = 2x = 20) were analyzed for the introgression of A. cardenasii chromosome segments. Seventy-three RFLP probes and 70 RAPD primers, expressing from one to four A. cardenasii-specific bands, were used to evaluate the set of introgression lines. Thirty-four RFLP probes and 45 RAPD primers identified putative A. cardenasii introgressed chromosome segments in one or more lines. Introgressed segments were detected by RFLP analysis in 10 of the 11 linkage groups; the smallest introgressed fragments were detected by single RFLP markers and the largest were detected by three or four adjacent markers and represented introgressed segments of 30-40 cM. Similar results were obtained with RAPD markers, although markers detecting introgressed fragments could not be placed on the peanut linkage map. Introgression into both A. hypogaea genomes was detected and its implication in breeding for disease resistance is discussed.
对46个来自花生（Arachis hypogaea L.，2n = 4x = 40）与卡氏花生（A. cardenasii Krapov. & W. C. Gregory，2n = 2x = 20）杂交的渐渗系（F10C9）进行了卡氏花生染色体片段渐渗分析。使用73个RFLP探针和70个RAPD引物（每个引物产生1至4条卡氏花生特异性条带）对该组渐渗系进行评估。34个RFLP探针和45个RAPD引物在一个或多个品系中鉴定出推定的卡氏花生渐渗染色体片段。通过RFLP分析在11个连锁群中的10个中检测到渐渗片段；最小的渐渗片段由单个RFLP标记检测到，最大的由三个或四个相邻标记检测到，代表30 - 40 cM的渐渗片段。使用RAPD标记也获得了类似结果，尽管检测到渐渗片段的标记无法定位到花生连锁图谱上。检测到向花生两个基因组的渐渗，并讨论了其在抗病育种中的意义。
