Utoguchi N, Dantakean A, Makimoto H, Wakai Y, Tsutsumi Y, Nakagawa S, Mayumi T
Faculty of Pharmaceutical Sciences, Osaka University.
Jpn J Cancer Res. 1995 Feb;86(2):193-201. doi: 10.1111/j.1349-7006.1995.tb03039.x.
Rat KMT-17 fibrosarcoma-derived endothelial cells were isolated by Percoll gradient centrifugation with an attaching-speed separation technique, and their properties in culture were examined. The primary cultured tumor-derived endothelial cells (TEC) showed angiotensin-converting enzyme activity, positivity for Factor VIII-related antigen staining, and typical capillary-like formation on Matrigel. The primary cultured TEC monolayer showed greater permeability than normal tissue-derived endothelial cell (aorta, vena cava and epididymal fat capillary) monolayers on FITC-dextran diffusion (molecular weight 70,000). Leukocyte adhesion to TEC was reduced compared to that to fat-derived capillary endothelial cells. These characteristics resembled those of tumor vascular endothelium, and were observed both in the primary and first-passage cell cultures, but not in the fourth-passage cell cultures. Our findings indicate that primary or subcultured TEC are applicable for studies of the physiological characteristics of tumor endothelial cells.
采用Percoll梯度离心结合贴壁速度分离技术分离大鼠KMT - 17纤维肉瘤来源的内皮细胞，并检测其体外培养特性。原代培养的肿瘤来源内皮细胞（TEC）显示出血管紧张素转换酶活性、因子VIII相关抗原染色阳性，以及在基质胶上形成典型的毛细血管样结构。在异硫氰酸荧光素标记葡聚糖（分子量70,000）扩散实验中，原代培养的TEC单层比正常组织来源的内皮细胞（主动脉、腔静脉和附睾脂肪毛细血管）单层具有更高的通透性。与脂肪来源的毛细血管内皮细胞相比，白细胞与TEC的黏附减少。这些特性与肿瘤血管内皮细胞相似，在原代和第一代传代细胞培养中均有观察到，但在第四代传代细胞培养中未观察到。我们的研究结果表明，原代或传代培养的TEC适用于肿瘤内皮细胞生理特性的研究。
