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Correlation of gene-specific damage with cisplatin between human adenocarcinoma cells and peripheral blood mononuclear cells analyzed by polymerase chain reaction-stop assay.通过聚合酶链反应终止法分析人腺癌细胞与外周血单个核细胞中基因特异性损伤与顺铂的相关性。
Jpn J Cancer Res. 1995 Feb;86(2):233-8. doi: 10.1111/j.1349-7006.1995.tb03044.x.
2
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本文引用的文献

1
Gene-specific damage produced by cisplatin, ormaplatin and UV light in human cells as assayed by the polymerase chain reaction.
Oncol Res. 1993;5(3):111-8.
2
Rapid polymerase chain reaction assay to detect variation in the extent of gene-specific damage between cisplatin- or VP-16-resistant and sensitive lung cancer cell lines.采用快速聚合酶链反应分析法检测顺铂或依托泊苷耐药及敏感肺癌细胞系之间基因特异性损伤程度的差异。
Jpn J Cancer Res. 1994 Jul;85(7):669-73. doi: 10.1111/j.1349-7006.1994.tb02412.x.
3
DNA repair in an active gene: removal of pyrimidine dimers from the DHFR gene of CHO cells is much more efficient than in the genome overall.活跃基因中的DNA修复：从CHO细胞的二氢叶酸还原酶（DHFR）基因中去除嘧啶二聚体比在整个基因组中更有效。
Cell. 1985 Feb;40(2):359-69. doi: 10.1016/0092-8674(85)90150-3.
4
Interindividual human variation in cisplatinum sensitivity, predictable in an in vitro assay?个体间对顺铂敏感性的差异，能否在体外试验中预测？
Mutat Res. 1987 Jan;190(1):59-62. doi: 10.1016/0165-7992(87)90083-2.
5
Survival of UV-irradiated mammalian cells correlates with efficient DNA repair in an essential gene.紫外线照射的哺乳动物细胞的存活与一个必需基因中的高效DNA修复相关。
Proc Natl Acad Sci U S A. 1986 Jun;83(11):3830-3. doi: 10.1073/pnas.83.11.3830.
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The formation, isolation and characterization of DNA adducts produced by anticancer platinum complexes.抗癌铂配合物产生的DNA加合物的形成、分离及特性分析
Pharmacol Ther. 1987;34(2):155-66. doi: 10.1016/0163-7258(87)90009-x.
7
Heterogeneous DNA damage and repair in the mammalian genome.
Cancer Res. 1987 Dec 15;47(24 Pt 1):6426-36.
8
Platinum-DNA adducts in leukocyte DNA correlate with disease response in ovarian cancer patients receiving platinum-based chemotherapy.接受铂类化疗的卵巢癌患者白细胞DNA中的铂-DNA加合物与疾病反应相关。
Proc Natl Acad Sci U S A. 1987 Jul;84(14):5024-8. doi: 10.1073/pnas.84.14.5024.
9
General method for quantifying base adducts in specific mammalian genes.定量特定哺乳动物基因中碱基加合物的通用方法。
Proc Natl Acad Sci U S A. 1988 Jun;85(11):3723-7. doi: 10.1073/pnas.85.11.3723.
10
The measurement of cisplatin-DNA adduct levels in testicular cancer patients.睾丸癌患者中顺铂-DNA加合物水平的测量。
Carcinogenesis. 1988 Oct;9(10):1909-11. doi: 10.1093/carcin/9.10.1909.

通过聚合酶链反应终止法分析人腺癌细胞与外周血单个核细胞中基因特异性损伤与顺铂的相关性。

Correlation of gene-specific damage with cisplatin between human adenocarcinoma cells and peripheral blood mononuclear cells analyzed by polymerase chain reaction-stop assay.

作者信息

Oshita F, Arioka H, Heike Y, Shiraishi J, Saijo N

机构信息

Division of Medical Oncology, National Cancer Center Hospital, Tokyo.

出版信息

Jpn J Cancer Res. 1995 Feb;86(2):233-8. doi: 10.1111/j.1349-7006.1995.tb03044.x.

DOI:10.1111/j.1349-7006.1995.tb03044.x
PMID:7730149
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5920763/
Abstract

We investigated gene-specific damage in adenocarcinoma cells, obtained from pleural effusions of 9 primary lung cancer patients, induced by incubation with cisplatin for 3 h in vitro. The 2.7 kb fragment of the hypoxanthine phosphoribosyltransferase (HPRT) gene was amplified by the polymerase chain reaction (PCR) to quantify the DNA damage. A 7-fold difference in the extent of gene-specific damage among the patients was observed. Mononuclear cells (MNC) were obtained from freshly isolated blood from the same patients before they received chemotherapy. These cells were also incubated with cisplatin in vitro, and PCR amplification of the HPRT gene was carried out. A 4-fold variation of DNA damage among the patients was observed. Moreover, there was a linear correlation between the extents of the DNA damage in the tumor cells and MNCs (R2 = 0.676, P = 0.0016). These results suggest that the PCR-stop assay could be used to detect interindividual variations in the extent of gene-specific damage in both tumor cells and MNC from the same patients induced by cisplatin treatment. In conclusion, MNC could be used to analyze cisplatin-induced gene-specific damage in cancer patients whose tumor cells are inaccessible.

摘要

我们研究了来自9例原发性肺癌患者胸腔积液的腺癌细胞在体外与顺铂孵育3小时后基因特异性损伤情况。通过聚合酶链反应（PCR）扩增次黄嘌呤磷酸核糖转移酶（HPRT）基因的2.7 kb片段，以定量DNA损伤。观察到患者之间基因特异性损伤程度存在7倍差异。单核细胞（MNC）取自同一患者化疗前新鲜分离的血液。这些细胞也在体外与顺铂孵育，并对HPRT基因进行PCR扩增。观察到患者之间DNA损伤存在4倍差异。此外，肿瘤细胞和MNC中的DNA损伤程度之间存在线性相关性（R2 = 0.676，P = 0.0016）。这些结果表明，PCR终止试验可用于检测同一患者的肿瘤细胞和MNC中顺铂治疗诱导的基因特异性损伤程度的个体间差异。总之，对于肿瘤细胞难以获取的癌症患者，MNC可用于分析顺铂诱导的基因特异性损伤。