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采用快速聚合酶链反应分析法检测顺铂或依托泊苷耐药及敏感肺癌细胞系之间基因特异性损伤程度的差异。

Rapid polymerase chain reaction assay to detect variation in the extent of gene-specific damage between cisplatin- or VP-16-resistant and sensitive lung cancer cell lines.

作者信息

Oshita F, Saijo N

机构信息

Division of Medical Oncology, National Cancer Center Hospital, Tokyo.

出版信息

Jpn J Cancer Res. 1994 Jul;85(7):669-73. doi: 10.1111/j.1349-7006.1994.tb02412.x.

DOI:10.1111/j.1349-7006.1994.tb02412.x
PMID:8071107
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5919540/
Abstract

We previously established a rapid and facile polymerase chain reaction (PCR)-stop assay for quantitation of specific gene damage in very small numbers of cells. The present study investigated whether the PCR-stop assay was able to detect variation in the extent of DNA damage in transcribed active genes between cisplatin- or VP-16-resistant and sensitive cells. The assay demonstrated that about twice as much genetic damage occurs in PC-9 cells than in cisplatin-resistant PC-9/CDDP cells following cisplatin exposure and about 4.6 times more damage occurs in H69 than in VP-16-resistant H69/VP cells following VP-16 exposure. These results show that DNA damage, as detected by PCR-stop assay, correlates with cytotoxicity. In conclusion, the PCR-stop assay could be useful in detecting variation in DNA damage in specific genes.

摘要

我们之前建立了一种快速简便的聚合酶链反应（PCR）终止法，用于定量极少量细胞中的特定基因损伤。本研究调查了PCR终止法是否能够检测顺铂或依托泊苷耐药和敏感细胞中转录活性基因的DNA损伤程度差异。该检测表明，顺铂处理后，PC-9细胞中的基因损伤约为顺铂耐药的PC-9/CDDP细胞的两倍，依托泊苷处理后，H69细胞中的损伤约为依托泊苷耐药的H69/VP细胞的4.6倍。这些结果表明，PCR终止法检测到的DNA损伤与细胞毒性相关。总之，PCR终止法可用于检测特定基因中DNA损伤的差异。

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本文引用的文献

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bcl-2 protein inhibits etoposide-induced apoptosis through its effects on events subsequent to topoisomerase II-induced DNA strand breaks and their repair.bcl-2蛋白通过影响拓扑异构酶II诱导的DNA链断裂及其修复后的事件，抑制依托泊苷诱导的细胞凋亡。
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Platinum-DNA adducts in leukocyte DNA correlate with disease response in ovarian cancer patients receiving platinum-based chemotherapy.接受铂类化疗的卵巢癌患者白细胞DNA中的铂-DNA加合物与疾病反应相关。
Proc Natl Acad Sci U S A. 1987 Jul;84(14):5024-8. doi: 10.1073/pnas.84.14.5024.
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