Fan M Z, Sauer W C, Hardin R T, Lien K A
Department of Animal Science, University of Alberta, Edmonton, Canada.
J Anim Sci. 1994 Nov;72(11):2851-9. doi: 10.2527/1994.72112851x.
Studies were carried out to investigate the effect of dietary amino acid level on apparent ileal amino acid digestibility. Six barrows, average initial BW 35 kg, were fitted with a simple T-cannula at the distal ileum and fed six diets according to a 6 x 6 Latin square design. Six cornstarch-based diets containing six levels of CP from SBM (4, 8, 12, 16, 20, and 24% CP, respectively) were formulated. Chronic oxide was included as a digestibility marker. Each experimental period consisted of 8 d. After a 6-d adaptation period, ileal digesta were collected for 24 h during d 7 and 9 at 2-h intervals. The pigs were fed twice daily, equal amounts, at 0800 and 2000. The dietary allowance was 1,600 g/d during the first period and increased by 100 g each following period. There was a quadratic increase (P < .05) in apparent ileal amino acid digestibility as the dietary CP content was increased from 4 to 24%. Initially, the apparent ileal amino acid digestibilities increased sharply then gradually reached their plateaus, after which there were no further increases and the digestibility values became independent of the dietary amino acid levels. The lower end points of 95% confidence intervals of the plateau ileal digestibility values were defined to be the initial plateau digestibilities. The dietary CP and amino acid contents, corresponding to the initial plateau digestibility values, represent the dietary threshold levels.(ABSTRACT TRUNCATED AT 250 WORDS)
开展了多项研究以调查日粮氨基酸水平对回肠氨基酸表观消化率的影响。选用6头平均初始体重35千克的公猪,在回肠末端安装简单的T型套管,并按照6×6拉丁方设计饲喂6种日粮。配制了6种以玉米淀粉为基础的日粮,分别含有6种不同水平的豆粕粗蛋白(分别为4%、8%、12%、16%、20%和24%粗蛋白)。添加了氧化铬作为消化率标记物。每个试验期为8天。经过6天的适应期后,在第7天和第9天每隔2小时收集24小时的回肠食糜。猪每天饲喂两次,量相等,分别在08:00和20:00。第一个时期的日粮供应量为1600克/天,随后每个时期增加100克。随着日粮粗蛋白含量从4%增加到24%,回肠氨基酸表观消化率呈二次增加(P<0.05)。最初,回肠氨基酸表观消化率急剧增加,然后逐渐达到平台期,此后不再增加,消化率值与日粮氨基酸水平无关。平台期回肠消化率值95%置信区间的下限被定义为初始平台期消化率。与初始平台期消化率值相对应的日粮粗蛋白和氨基酸含量代表日粮阈值水平。(摘要截断于250字)