果蝇类驱动蛋白KLP3A是中心纺锤体组装和胞质分裂起始所需的中间体成分。
The Drosophila kinesin-like protein KLP3A is a midbody component required for central spindle assembly and initiation of cytokinesis.
作者信息
Williams B C, Riedy M F, Williams E V, Gatti M, Goldberg M L
机构信息
Section of Genetics and Development, Cornell University, Ithaca, New York 14853-2703, USA.
出版信息
J Cell Biol. 1995 May;129(3):709-23. doi: 10.1083/jcb.129.3.709.
Abstract
We describe here a new member of the kinesin superfamily in Drosophila, KLP3A (Kinesin-Like-Protein-at-3A). The KLP3A protein localizes to the equator of the central spindle during late anaphase and telophase of male meiosis. Mutations in the KLP3A gene disrupt the interdigitation of microtubules in spermatocyte central spindles. Despite this defect, anaphase B spindle elongation is not obviously aberrant. However, cytokinesis frequently fails after both meiotic divisions in mutant testes. Together, these findings strongly suggest that the KLP3A presumptive motor protein is a critical component in the establishment or stabilization of the central spindle. Furthermore, these results imply that the central spindle is the source of signals that initiate the cleavage furrow in higher cells.
摘要
我们在此描述果蝇中驱动蛋白超家族的一个新成员，KLP3A（位于3A位点的类驱动蛋白）。在雄性减数分裂的后期和末期，KLP3A蛋白定位于纺锤体中央区的赤道面。KLP3A基因突变会破坏精母细胞纺锤体中央区微管的相互交错。尽管存在这一缺陷，但后期B纺锤体伸长并无明显异常。然而，在突变型睾丸的两次减数分裂后，胞质分裂常常失败。这些发现共同强烈表明，KLP3A推定的运动蛋白是纺锤体中央区建立或稳定的关键组成部分。此外，这些结果意味着纺锤体中央区是启动高等细胞中分裂沟信号的来源。
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