一种用于检测组织培养上清液和活检样本中山羊痘病毒的抗原捕获酶联免疫吸附测定法。

An antigen trapping ELISA for the detection of capripoxvirus in tissue culture supernatant and biopsy samples.

作者信息

Carn V M

机构信息

Institute for Animal Health, Pirbright Laboratory, Woking, UK.

出版信息

J Virol Methods. 1995 Jan;51(1):95-102. doi: 10.1016/0166-0934(94)00138-7.

DOI:10.1016/0166-0934(94)00138-7

PMID:7730442

Abstract

A trapping ELISA for the detection of capripoxvirus antigen in tissue culture supernatant and biopsy material was developed, using a guinea-pig polyclonal detector antiserum raised against a recombinant capripoxvirus specific antigen, expressed in Escherichia coli using the plasmid vector pGEX-2T. The ELISA detected antigen in tissue culture samples that on virus titration contained equal to or in excess of 10(2.8) TCID50/ml. Virus isolation and ELISA were compared for the detection of capripoxvirus in skin biopsy samples from sheep, goats and cattle. The ELISA compared well with virus isolation, and has applications as a diagnostic test. This assay reduces the reliance of diagnostic laboratories on tissue culture facilities, and can be used to confirm the presence of capripoxvirus in tissue culture.

摘要

利用针对在大肠杆菌中使用质粒载体pGEX - 2T表达的重组山羊痘病毒特异性抗原制备的豚鼠多克隆检测抗血清，开发了一种用于检测组织培养上清液和活检材料中山羊痘病毒抗原的捕获ELISA。该ELISA可检测组织培养样品中的抗原，这些样品经病毒滴定后每毫升含有等于或超过10(2.8) TCID50的病毒。对病毒分离法和ELISA法检测绵羊、山羊和牛皮肤活检样品中山羊痘病毒的效果进行了比较。ELISA法与病毒分离法效果相当，可作为一种诊断检测方法应用。该检测方法减少了诊断实验室对组织培养设施的依赖，可用于确认组织培养物中山羊痘病毒的存在。