通过免疫荧光和免疫电子显微镜对野生型和Thy 1突变细胞中T25糖蛋白进行定位。
Localization of T25 glycoprotein in wild-type and Thy 1- mutant cells by immunofluorescence and immunoelectron microscopy.
作者信息
Bourguignon L Y, Hyman R, Trowbridge I, Singer S J
出版信息
J Exp Med. 1978 May 1;147(5):1348-54. doi: 10.1084/jem.147.5.1348.
Abstract
The wild-type BW5147 (Thy 1+) cell line and its Thy 1- mutant derivative BW5147 (Thy 1-a) were examined by immunofluorescence and immunoelectron microscopy for the presence of T25, the glycoprotein which bears the Thy 1 alloantigen. The wild-type cell had T25 predominantly localized on the cell surface. In the mutant cell line, T25 accumulated intracellularly and was present in a clustered distribution throughout the cytoplasm. T25 was not present on the surface of the mutant cell line in significant amount.
摘要
通过免疫荧光和免疫电子显微镜检查野生型BW5147（Thy 1+）细胞系及其Thy 1-突变衍生物BW5147（Thy 1-a），以检测携带Thy 1同种异体抗原的糖蛋白T25的存在情况。野生型细胞中T25主要定位于细胞表面。在突变细胞系中，T25在细胞内积累，并以聚集分布存在于整个细胞质中。突变细胞系表面不存在大量的T25。
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