Sun H, Jacobs S C, Smith G L, Dixon L K, Parkhouse R M
BBSRC Institute for Animal Health, Pirbright Laboratory, Woking, UK.
J Gen Virol. 1995 May;76 ( Pt 5):1117-27. doi: 10.1099/0022-1317-76-5-1117.
The African swine fever virus (ASFV) j13L gene encodes a 177 amino acid protein (19.0 kDa) with a putative transmembrane domain between residues 32 and 52. There is a potential signal peptide cleavage site at residue 54 and several possible motifs for phosphorylation and myristylation. Rabbit antisera raised against a synthetic peptide from the C terminus of the j13L ORF identified proteins of 25-27 kDa in cells infected with a recombinant vaccinia virus expressing the j13L ORF, in ASFV-infected cells and in purified extracellular ASF virions. In ASFV-infected cells the j13L protein was expressed late during infection and exhibited size variation (25-27 kDa) between the different ASFV strains. Nucleotide sequence analysis of the gene in these strains showed that these size differences were due to variation in the number and sequence of tandemly repeated amino acid repeats. Although ASFV-infected animals made antibodies to the j13L protein, no protection was observed when pigs were vaccinated with a recombinant vaccinia virus expressing the j13L ORF.
非洲猪瘟病毒（ASFV）的j13L基因编码一种含177个氨基酸的蛋白质（19.0 kDa），在第32至52位氨基酸之间有一个推定的跨膜结构域。在第54位氨基酸处有一个潜在的信号肽切割位点，还有几个可能的磷酸化和豆蔻酰化基序。用针对j13L开放阅读框（ORF）C端合成肽制备的兔抗血清，在感染表达j13L ORF的重组痘苗病毒的细胞、ASFV感染的细胞以及纯化的细胞外ASFV病毒粒子中，鉴定出了25 - 27 kDa的蛋白质。在ASFV感染的细胞中，j13L蛋白在感染后期表达，并且在不同的ASFV毒株之间表现出大小差异（25 - 27 kDa）。对这些毒株中该基因的核苷酸序列分析表明，这些大小差异是由于串联重复氨基酸重复序列的数量和序列变化所致。尽管感染ASFV的动物会产生针对j13L蛋白的抗体，但用表达j13L ORF的重组痘苗病毒对猪进行疫苗接种时，未观察到保护作用。
