Sun H, Jenson J, Dixon L K, Parkhouse M E
Institute for Animal Health, Pirbright Laboratory, Surrey, UK.
J Gen Virol. 1996 May;77 ( Pt 5):941-6. doi: 10.1099/0022-1317-77-5-941.
The African swine fever virus (ASFV) open reading frame (ORF) that is named jl8L in the Malawi (LIL20/1) isolate and E199L in the Ba71V isolate encodes a cysteine rich protein of 195 amino acids with a predicted molecular mass of 21.7 kDa and a hydrophobic domain near the C terminus. There are several possible motifs for glycosylation, phosphorylation and myristoylation. Rabbit antisera and monoclonal antibodies raised against a recombinant ASFV j18L protein expressed as a fusion protein with glutathione S-transferase (GST) identified proteins of 19.0-20 kDa in cells infected with different ASFV strains and with a recombinant vaccinia virus expressing j18L. The monoclonal antibodies detected a protein of 20.0 kDa whereas rabbit antisera detected two proteins with relative molecular masses of 15.0 and 20.0 kDa in purified extracellular ASF virions. In ASFV-infected cells, the j18L protein was expressed late post-infection and was localized mainly in the viral factories.
在马拉维（LIL20/1）分离株中被命名为jl8L且在Ba71V分离株中被命名为E199L的非洲猪瘟病毒（ASFV）开放阅读框（ORF）编码一种富含半胱氨酸的蛋白质，该蛋白质由195个氨基酸组成，预测分子量为21.7 kDa，且在C端附近有一个疏水区。存在几种可能的糖基化、磷酸化和豆蔻酰化基序。用针对与谷胱甘肽S-转移酶（GST）融合表达的重组ASFV j18L蛋白制备的兔抗血清和单克隆抗体，在感染不同ASFV毒株以及感染表达j18L的重组痘苗病毒的细胞中鉴定出了分子量为19.0 - 20 kDa的蛋白质。单克隆抗体检测到一种分子量为20.0 kDa的蛋白质，而兔抗血清在纯化的细胞外非洲猪瘟病毒粒子中检测到两种相对分子量分别为15.0和20.0 kDa的蛋白质。在感染ASFV的细胞中，j18L蛋白在感染后期表达，且主要定位于病毒工厂。
