三个非洲猪瘟病毒基因，其编码的蛋白质与痘苗病毒假定解旋酶具有同源性。

Three African swine fever virus genes encoding proteins with homology to putative helicases of vaccinia virus.

作者信息

Baylis S A, Twigg S R, Vydelingum S, Dixon L K, Smith G L

机构信息

Sir William Dunn School of Pathology, University of Oxford, U.K.

出版信息

J Gen Virol. 1993 Sep;74 ( Pt 9):1969-74. doi: 10.1099/0022-1317-74-9-1969.

DOI:10.1099/0022-1317-74-9-1969

PMID:8376971

Abstract

Sequence analysis of the SalI g, h, i and j restriction fragments of the African swine fever virus (ASFV) genome from the virulent isolate Malawi (LIL20/1) identified three open reading frames (ORFs) encoding predicted proteins of 125.0K (g10L), 80.4K (j10L) and 58.0K (j11L) which showed homology to members of the DNA and RNA helicase superfamily. ORFj10L was related to protein 4 of the Kluyveromyces lactis killer plasmid pKG12 and to two putative helicases, D6R and D11L, of vaccinia virus. ORF g10L was most closely related to ASFVj10L and to vaccinia virus D11L. ORFj11L was homologous to A18R, a third putative helicase of vaccinia virus. The possible functions of these genes in the replication of ASFV are discussed and the evolutionary implications are considered.

摘要

对来自强毒株马拉维（LIL20/1）的非洲猪瘟病毒（ASFV）基因组的SalI g、h、i和j限制性片段进行序列分析，鉴定出三个开放阅读框（ORF），它们编码预测的蛋白质，分子量分别为125.0K（g10L）、80.4K（j10L）和58.0K（j11L），这些蛋白质与DNA和RNA解旋酶超家族的成员具有同源性。ORFj10L与乳酸克鲁维酵母杀伤质粒pKG12的蛋白质4以及痘苗病毒的两个推定解旋酶D6R和D11L相关。ORF g10L与ASFVj10L以及痘苗病毒D11L关系最为密切。ORFj11L与痘苗病毒的第三个推定解旋酶A18R同源。讨论了这些基因在ASFV复制中的可能功能，并考虑了其进化意义。