Turesson I
Acta Med Scand. 1976;199(4):293-304. doi: 10.1111/j.0954-6820.1976.tb06735.x.
Cell suspensions of human bone marrow, spleen, lymph nodes and palatine tonsils have been investigated for the presence of intracellular immunoglobulins by a direct immunofluorescence technique, using monospecific antisera against human Ig heavy chains alpha, mu and gamma and light chains kappa and lambda. Serum Ig levels were determined and the number of positive cells was compared with the concentration and calculated synthetic rate of serum Ig in each individual. The 28 patients studied covered a wide range of diagnoses and included those with normal as well as pathologically decreased or increased serum Ig levels. There was a high correlation between the calculated synthetic rate of each Ig class and the percentage of cells positive for the same Ig class in the bone marrow but not in the spleen, lymph nodes or tonsils. The Ig-containing cells constituted a much larger proportion of the total lymphoid cell population in the bone marrow than in the peripheral lymphoid organs. The estimated total number of Ig-containing cells was also much larger in the bone marrow than in the other organs investigated. It is concluded that in man the bone marrow is the major site of serum Ig synthesis and that the average synthetic rate per cell is the same regardless which of the three major Ig classes is produced. The role played by different lymphoid organs in humoral immunity is discussed in the light of the present results and reported data regarding lymphocyte and plasma cell kinetics in mammals.
利用抗人Ig重链α、μ和γ以及轻链κ和λ的单特异性抗血清，通过直接免疫荧光技术，对人骨髓、脾脏、淋巴结和腭扁桃体的细胞悬液进行了细胞内免疫球蛋白存在情况的研究。测定了血清Ig水平，并将阳性细胞数与每个个体血清Ig的浓度和计算出的合成率进行了比较。所研究的28例患者涵盖了广泛的诊断范围，包括血清Ig水平正常以及病理性降低或升高的患者。在骨髓中，每种Ig类别的计算合成率与同一Ig类别的阳性细胞百分比之间存在高度相关性，但在脾脏、淋巴结或扁桃体中则不存在这种相关性。含Ig细胞在骨髓中占总淋巴细胞群体的比例远大于外周淋巴器官。骨髓中含Ig细胞的估计总数也远大于其他所研究的器官。结论是，在人类中，骨髓是血清Ig合成的主要部位，并且无论产生三种主要Ig类别中的哪一种，每个细胞的平均合成率都是相同的。根据目前的结果以及有关哺乳动物淋巴细胞和浆细胞动力学的报道数据，讨论了不同淋巴器官在体液免疫中所起的作用。
