Turesson I
Acta Med Scand. 1978;203(4):247-55. doi: 10.1111/j.0954-6820.1978.tb14868.x.
Cell suspensions of bone marrow and lymphoid tissue from 85 patients with monoclonal gammapathy were investigated by a direct immunofluorescence procedure for the detection of intracellular immunoglobulin alpha, mu, gamma, kappa and lambda chains. Serum Ig levels were determined and daily syntheric rates estimated. In all cases the majority of Ig-containing bone marrow cells contained the same Ig class as that of the M-component in serum or urine indicating a diffuse distriubtion of these clones in the bone marrow. This was observed not only in myeloma but also in benign monoclonal gammapathy (BMG) and lymphoma with an M-component. The M-component producing clone could be traced to extramedullary lymphoid tissue in myeloma but usually not in BMG. A positive correlation was found between the calculated synthetic rate of the M-component and the number of Ig-containing cells in the bone marrow and some indication was found that the synthetic rate per cell might be lower in IgM and IgG than in IgA monoclonal gammapathy. The depressed level of polyclonal Ig in myeloma and to some extent in BMG was parallelled by a diminished number of Ig-containing cells in the bone marrow.
采用直接免疫荧光法对85例单克隆丙种球蛋白病患者的骨髓和淋巴组织细胞悬液进行检测，以检测细胞内免疫球蛋白α、μ、γ、κ和λ链。测定血清Ig水平并估算每日合成率。在所有病例中，大多数含Ig的骨髓细胞所含的Ig类别与血清或尿液中的M成分相同，这表明这些克隆在骨髓中呈弥漫性分布。不仅在骨髓瘤中观察到这种情况，在良性单克隆丙种球蛋白病（BMG）和伴有M成分的淋巴瘤中也观察到了。产生M成分的克隆在骨髓瘤中可追溯到髓外淋巴组织，但在BMG中通常不能追溯到。发现M成分的计算合成率与骨髓中含Ig细胞的数量之间存在正相关，并且发现有迹象表明，在IgM和IgG单克隆丙种球蛋白病中，每个细胞的合成率可能低于IgA单克隆丙种球蛋白病。骨髓瘤以及在一定程度上BMG中多克隆Ig水平的降低与骨髓中含Ig细胞数量的减少相平行。
