两种血清学检测方法诊断麻风病患者的性能研究。

A study on performance of two serological assays for diagnosis of leprosy patients.

作者信息

Parkash O, Chaturvedi V, Girdhar B K, Sengupta U

机构信息

Central JALMA Institute for Leprosy, Tajganj, Agra, India.

出版信息

Lepr Rev. 1995 Mar;66(1):26-30. doi: 10.5935/0305-7518.19950004.

DOI:10.5935/0305-7518.19950004

PMID:7731338

Abstract

We compared 2 serological tests for the diagnosis of leprosy to test their performances. The tests include the serum antibody competition test (SACT) for the detection of antibodies to Mycobacterium leprae-specific epitope on 35 KDa protein molecule, and M. leprae gelatin particle agglutination assay (MLPA), for the detection of antiphenolic glycolipid-1 (PGL-1) antibodies. In both the assays a higher serological positivity was seen amongst multibacillary (MB) patients than those in paucibacillary (PB) patients. Taking all leprosy patients together, the sensitivity of SACT (59.7%) was observed to be statistically comparable to that of MLPA (66.9%). However, SACT proved to be more specific (97.7%) than MLPA (75.0%). The agreement between these 2 assays was observed to be moderate.

摘要

我们比较了两种用于麻风病诊断的血清学检测方法，以测试它们的性能。这些检测包括血清抗体竞争试验（SACT），用于检测针对35 kDa蛋白分子上麻风分枝杆菌特异性表位的抗体，以及麻风杆菌明胶颗粒凝集试验（MLPA），用于检测抗酚糖脂-1（PGL-1）抗体。在这两种检测中，多菌型（MB）患者的血清学阳性率高于少菌型（PB）患者。将所有麻风病患者综合来看，血清抗体竞争试验（SACT）的敏感性（59.7%）在统计学上与麻风杆菌明胶颗粒凝集试验（MLPA）（66.9%）相当。然而，血清抗体竞争试验（SACT）被证明比麻风杆菌明胶颗粒凝集试验（MLPA）（75.0%）更具特异性（97.7%）。观察到这两种检测方法之间的一致性为中等。