Higashino F, Yoshida K, Noumi T, Seiki M, Fujinaga K
Department of Molecular Biology, Sapporo Medical University, School of Medicine, Japan.
Oncogene. 1995 Apr 6;10(7):1461-3.
An Ets-related E1A-F has been characterized as an enhancer-binding protein for the adenovirus E1A gene. Here we show, in transient expression assays, that E1A-F can activate three different subclasses of the matrix metalloproteinase gene promoters. Expressions of the chloramphenicol acetyltransferase (CAT) reporter gene under the control of stromelysin, type I collagenase and 92 kD type IV collagenase promoters were increased approximately 10- to 20-fold by co-transfection with the E1A-F expression vector. Activation levels were as much high as those obtained by exogenous expression of AP-1 transcription factor. These results suggest that E1A-F positively regulates transcriptions from matrix metalloproteinase genes that are associated with invasion and metastasis of tumor cells.
一种与Ets相关的E1A-F已被鉴定为腺病毒E1A基因的增强子结合蛋白。在此，我们在瞬时表达试验中表明，E1A-F可激活基质金属蛋白酶基因启动子的三种不同亚类。在基质溶解素、I型胶原酶和92kD IV型胶原酶启动子控制下的氯霉素乙酰转移酶（CAT）报告基因的表达，通过与E1A-F表达载体共转染而增加了约10至20倍。激活水平与通过AP-1转录因子的外源表达所获得的水平一样高。这些结果表明，E1A-F正向调节与肿瘤细胞侵袭和转移相关的基质金属蛋白酶基因的转录。
